微生物酶解破壁提制葛根黄酮的工艺.pdf

28 2011, Vol. 32, No. 02 食品科学 ※工艺技术 微生物酶解破壁提制葛根黄酮的工艺 1,2 1 1 1 1 王星敏 ，殷钟意 ，郑旭煦 ，张渝文 ，曾 乐 (1.重庆工商大学环境与生物工程学院，重庆 400067 ；2 .重庆大学资源及环境科学学院，重庆 400033) 摘 要：采用生物酶促发酵降解葛根废渣的植物组织，有效提取葛根黄酮，通过高效液相色谱法测定黄酮的含量， 优选出发酵菌种，对比葛渣不同处理方式，对接种量、培养基质和不同工艺参数等进行优化，并采用超声波萃取 得到葛根黄酮粗品。结果表明：黑曲霉好氧发酵处理葛根废渣3.5g 、添加辅料豆粕1.0g 、葛根废渣质量与水的体 积比1:20(g/mL) 、黑曲霉接入量5% 、pH 5 、在30 ℃发酵60h ，测得葛根黄酮含量为3.769 μg/mL ；经超声波协同 处理5min 后，葛根黄酮质量浓度为5.204 μg/mL ，经分离、蒸馏后获得葛根黄酮粗品2 .974mg/g 。 关键词：酶促反应；葛根黄酮；破壁；葛根废渣 Microbial Fermentation for Cell Disruption in Combination with Ultrasonic Treatment for the Extraction of Total Flavonoids from Pueraria lobata Tubers 1,2 1 1 1 1 WAN G Xing-min ，YIN Zhon g-y i ，ZHENG Xu-xu ，ZHANG Yu-wen ，ZENG Le (1. College of Environmental and Biological Engineering, Chongqing Technology and Business University, Chongqing 400067, China ；2. College of Resources and Environmental Science, Chongqing University, Chongqing 400033, China) Abstract：Some enzymes produced during mold fermentation degrade the tissue structures of the residue left after the extraction of starch from Pueraria lobata tubers to facilitate total flavonoids extraction. On the basis of the fact, the development of a method for the extraction of total flavonoids from the residue left after starch extraction u sing mold fermentation and sub sequent ultrasonic treatment was presented in this work. Asp ergillus niger and Trichoderma koningii were separately cultured in Pueraria l