丝胶蛋白的细胞相容性与抗菌性能研究.PDF

Vol.49 第49卷 No.11, Nov. 2012 2012年11月 第11期 研究与技术 丝胶蛋白的细胞相容性和抗菌性能研究 陈忠敏，罗 琴，张瑶琴，刘 琼 (重庆理工大学 药学与生物工程学院，重庆 400054) 摘要：研究了从茧壳、茧衣中提取丝胶蛋白(S1、S2)及茧壳丝胶进行酶解制备的小分子丝胶蛋白肽(S3) 的细胞相容性，以及对革兰 氏阴性菌、阳性菌的抗菌作用。结果表明：三种丝胶蛋白均“对细胞无毒性”，并随丝胶浓度的增大对细胞生长有促进作用。采用 细菌生长抑制试验定量测试了各梯度浓度下丝胶的抑菌率，结果显示丝胶具有显著的抑菌作用，对金黄色葡萄球菌和的大肠杆菌抑 制率由大到小依次为S3、S1、S2和S2、S3、S1，丝胶浓度对抑菌性有一定影响。研究结果表明丝胶是一种具有良好抑菌性能的生 物相容性材料。 关键词：丝胶蛋白；细胞相容性；抗菌性 中图分类号：TS146 ；R318 文献标志码：A 文章编号：1001-7003(2012)11-0001-05 Cytocompatibility and antibacterial properties of sericin proteins CHEN Zhong-min, LUO Qin, ZHANG Yao-qin, LIU Qiong (College of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China) Abstract: This paper studies the cytocompatibility of sericin proteins (S1, S2) extracted from cocoon shell and cocoon outer floss respectively and small molecule sericin protein peptide (S3) prepared by enzymolysis of cocoon shell sericin, and studies the antibacterial properties against Gram-negative bacteria and Gram- positive bacteria. Results show that: all these three kinds of sericin protein have not toxicity to cells, and show the function of promoting cell growth with the increase of sericin concentration. Have a quantitative test of bacterial inhibition rate of sericin under each concentration gradient by using bacterial growth inhibition test, and results show that sericin has significant antibacterial properties, and inhibition rates of Staphylococcus aureus and Escherichia coli in descendin