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SSR和ISSR标记技术应用进展_蒋彩虹.pdf

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SSR和ISSR标记技术应用进展_蒋彩虹

2007 28 2 1-5 1 SSR ISSR * 266101 SSR ISSR SSRISSR S572.035 A 1007 02-0001-05 Application Advance of Molecular Marker Techniques of SSR and ISSR * JIANG Caihong, WANG Yuanying , SUN Yuhe (Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China) Abstract: The principle and characteristics of the molecular marker techniques of SSR and ISSR were described. The advances in their application to various genetic and breeding studies, including genetic diversity analyses, fingering maps and purity control of cultivars, gene localization, and marker-assisted selection were reviewed. The potential application in tobacco was suggested. Keywords: SSR; ISSR; molecular marker; tobacco DNA DNA SSR Moore 1991 simple sequence repeats, SSR microsatellite DNA 14 100200 bp SSR PCR [1] DNA SSR [2] 1.1.1 SSR SSR [3] RFLP AFLP RAPD SSRISSR SSR 1050 kb RAPD SSR 1 SSR ISSR SSR SSR ISSR [4] 1 SSR ISSR

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