赵祥强 矮泰引_3中半矮秆基因的分子定位.pdf

　遗 传 学 报 　Acta Genetica Sinica , February 2005 , 32 (2) : 189～196 ISSN 0379 - 4172 Mole c ula r Map pi n g of Tw o S e mi dw a rf Ge ne s i n a n i n dic a Ric e Va rie t y Ait ai yi n3 ( Or yz a s ati va L . ) ① ZHAO XiangQiang , L IAN G GuoHua , ZHOU JinSong , YAN ChangJie , CAO XiaoYing , GU MingHong ( The A gricult ural College , Yangz hou U niversity , Yangz hou 　225009 , Chi na) Abs t ra ct : The rice dwarf variety Aitaiyin3 is derived from a semidwarf cultivar Taiyin1. Genetic analysis indicated that the dwarf phenotype in Aitaiyin3 is involved in two recessive loci. Using bulk analysis with SSR markers ,the two loci were located on the chromosome 1 and chromosome 4 ,respectively. Moreover ,the allelism test showed that the locus on the chromosome 1 is allelic to the semidwarf gene sd1 ,while the other one is a new locus ,here was named as sdt2 . Further molecular mapping showed that sd1 was linked with SSR markers in the order of RM297RM302RM212OSR3 sd1 with genetic distances of 4 7 cM ,0 cM ,0 8 cM and 0 cM ,respectively. The linkage relationship of sdt2 with five SSR markers was SSR332RM1305 sdt2RM5633RM307RM401 with genetic distances of 11. 6 cM ,3 . 8 cM ,0 . 4 cM ,0 cM and 0 . 4 cM ,respectively. Ke y w or ds : rice ; semidwarf gene ; SSR markers ; molecular mapping 矮泰引3 中半矮秆基因的分子定位 赵祥强 , 梁国华 ①, 周劲松 , 严长杰 , 曹小迎 , 顾铭洪 (扬州大学农学院 , 扬州　225009) 摘 　要 : 矮泰引3 的矮生性状受两对独立遗传的半矮秆基因控制 ,利用 SS