水稻颖壳和浆片异常突变体ahl的基因定位.pdf

作物学报 ACTA AGRONOMICA SINICA 2011, 37(4): 629634 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2011.00629 水稻颖壳和浆片异常突变体ahl 的基因定位 王 增 李云峰** 马 娇 任德勇 王德仲 游小庆 桑贤春 何光华* / , 400716 : ahl (abnormal hull and lodicule), 10 EMS , , , 1 , (bulked segregation analysis, BSA)AHL 2 SSR RM14153 RM14167 , 1.19 cM 1.34 cM, 226 kb AHL : ; ; ; Gene Mapping of a Novel Mutant ahl in Rice ** WANG Zeng, LI Yun-Feng , MA Jiao, REN De-Yong, WANG De-Zhong, YOU Xiao-Qing, SANG * Xian-Chun, and HE Guang-Hua Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing 400716, China Abstract: Researches on rice flower development gene are very important in molecular breeding of rice for related traits. A rice flower mutant abnormal hull and lodicule (ahl) was identified from Jinhui 10 (Oryza sativa L. ssp. indica) treated by EMS. In ahl mutant, lemma and palea minished and significantly twisted along with the apparently elongated apical lodicules. This minished lemma and palea led to abnormal kernels after grain filling, meanwhile, the 1000-grain weight decreased. The trait was steadily inherited, and controlled by a pair of recessive gene. Using bulked segregation analysis method AHL was restricted between SSR markers RM14153 and RM14167, with genetic distances of 1.34 cM and 1.19 cM, respectively, which covered an approximate 226 kb region on the chromosome 2. The result is useful for further map-based cloning and functional analysis of AHL gene. Keywords: Ric