植物病毒病害人工miRNA自动应答防治技术.pdfVIP

因GUS 的高量表达，与前面的定量分析实验结果相映。综上所述，推测06GP1967 启动子受病毒诱导启动。 为了获得构建烟草病毒自动应答体系，需要获得具有正常功能的烟草天然 miRNA。本研究通过生物信息学方法，预测了可能具有生物学功能的烟草 miRNA， 找到烟草中的天然miRNA167前体序列。我们发现，过表达miRNA167的转基因烟 草植株，其靶基因ARF8表达水平受到明显抑制。形态观察结果显示，转基因植株虽 然叶片黄化，但是可以正常的开花结果。本实验找到了可以被病毒特异诱导的高表达 诱导型启动子06GP1967，还对烟草内源miRNA167进行了功能验证，为下一步构建 烟草病毒自动应答防治技术奠定了基础。 关键词：植物病毒；人工miRNA；启动子；烟草 II 万方数据 Abstract Viruse often cause plant abnormal growth and development, even death. In recent years, artificial miRNA technology for control of plant viru disease ha made ​​great achievements, often involved foreign artificial miRNA expre ion driven by a strong promoter in the plant. Those studie alway choose a strong constitutive promoter. The transgeneunder controlof a constitutivepromoter canbe expre ed stablyand efficiently at different developmental stages.However,high-level expre ion of foreign gene inthe recipient plant often turn to be a burden of plant and leading to abnormal growth and development of receptor plants. Hence, the vector which contain promoter induced by plant viruse and artificial miRNA constructed by endogenesi miRNA i beneficial to enhancethe effect ofdefensingplantviruses.Inthi experiment,themodelplanttobacco wa investigated. We try to find the promoter induced by plant viruse and endogenesi miRNApo e ed of complete function in tobacco, which perhap provide a theoretical basi forthepreventionandtreatmentofplantviru diseases. Thi research found 10candidate gene inducedby viru intobacco via conculting journal and using BLASTX in order to identifying gene involved in viru resistance response of tobacco. Tobacco under three viruse (Tobacco mosaic virus, Cucumber mosaic virus,Potato viru