槲皮素抗动脉粥样硬化的作用及机制.pdfVIP

R4蛋白表达均有差异，其表达水平均呈不同程度下调 （P0.05 ）。低剂量、 中剂量和高剂量在不同时间 HMGB1 、TLR4蛋白表达有差异 （P0.05 ）， 呈时间依赖性。结论：1.成功建立动脉粥样硬化的 ApoE-/-小鼠模型，为进 一步探讨槲皮素抗动脉粥样硬化的作用及机制奠定了基础。2.槲皮素具有 抗动脉粥样作用，其作用与药物剂量、时间具有相关性。3.HMGB1 、TL R4 的蛋白和基因表达下调，可能是槲皮素抗动脉粥样的分子机制。 关键词：槲皮素；动脉粥样硬化；高迁移率族蛋白 1；Toll 样受体 4 5 The effect and mechanism of quercetin on atherosclerosis in -/- ApoE mouse Abstract:Objecivet: to investigate the effect and mechanism of anti-AS of quercetin Methods: 1. to establish t`animal model of anti-AS of quercetin (1) dividing 46 normal-diet raised ApoE-/- mouse into five groups, each group containing eight mouse of 4 to 6-week-old. The five groups are: normal-diet group(3), control group(13), experimental group of low-dose quercetin (50mg/kg/d), median-dose quercetin (100mg/kg/d) and high-dose quercetin (50mg/kg/d),which have 10 mice in each group. (2) except the normal-diet group, all other groups are treated with high-fat diet for a period of 12 weeks. Then, 3 mice were killed in each group to investigate the AS by the method of HE staining, transmissionelectronmicroscope and blood cholesterol. 2. to investigate the expression of HMGB1 and TLR4 in gene and protein by the method of immunohistochemical and RT-PCR. 3. to investigate the expression of HMGB1 and TLR4 protein in blood by ELISA. Results: 1.after 12 weeks of high-fat diet, the HE staining implies the thickness, plague formation and cholesterol crystal in intima of aorta and transmissionelectronmicroscope examination, aortic endothelial cells are swollen, the cell membrane is damaged and the structure of perinuclear space is expanded. Th