模板法合成多孔硅胶整体柱及其色谱应用的探讨.pdfVIP

ABSTRACT Monolithic silica column is the core and motivation of development in chromatography. Monolith column with low column back pressure, high permeability, high mechanical strength ，good performance and strong column stability has attracted attentions since 1990s,as a new type of chromatography column ．It has been widely used in the fields of medicine, food and health. However, the preparation process is relatively difficult, until now only a few groups have reported it ．Therefore，it is necessary to find an appropriate method about preparation and modification of the monolithic silica column ． The development of the chromatography column was reviewed at first, and then we place importance on the points as follows: （1 ） The preparation of polystyrene microsphere. The polymerization microspheres is prepared by using emulsion polymerization and dispersion respectively, and then PS microspheres which have monodispersity and diameter of 200 nm and 2.6μm are obtained through the adjustment of the parameters. （2 ） The optimization of matrix of silica monolith. We used Methyl hydrogen silicone oil(PHMS) and Tetravinyl tetramethylcyelo tetrasiloxane(D Vi)as 4 crosslinking monomer, with methyl vinyl siloxane ligand platinum as catalyst to synthetise silica monolith. We made research into the ratio of PHMS and D Vi ， 4 catalyst content and crosslinking temperature on the influence of the substrate. The scanning electron microscopy (SEM) and optical images were used to characterize the macroscopic and microsco