冰冻红细胞解冻洗涤液的改良性研究.pdfVIP

.149. 临床输血与检验 2016年4 月第18 卷第2 期 冰冻红细胞解冻洗涤液的改良性研究 于来水康炜宫本兰叶萍张薇张磊梁晓华 【摘要】 目的探究在冰冻红细胞解冻洗涤液中添加适宜浓度的木糖醇，提高解冻红细胞回收率的可行性。方法选择 6 d 内制备的400 ml去白细胞悬浮红细胞20袋，离心去浆后分装等量 (100 ml) 浓缩红细胞作为实验组和对照组，实验组在 解冻第1 次洗涤液 (80 ml 9% NaCI)和第2次洗涤液 (200 ml 0.9% NaCI)中分别加入终浓度为1.2%、 0.2%的木糖醇，比 较两组的RBC回收率、红细胞渗透脆性、甘油残留量，同时测定冰冻红细胞解冻洗涤过程中各阶段的游离血红蛋白含量， 明确提高解冻红细胞回收率的关键步骤。结果实验组RBC回收率显著高于对照组 (t= 一 10.222. PO.O日，而红细胞渗 透脆性、甘油残留量与对照组相比差异无统计学意义 (P0.05) 。解冻第l 次洗涤后血浆游离血红蛋白含量以实验组显著 低于对照组 (t=24.4 96. P0.05) • 其余洗涤步骤的差异则无统计学意义 (P0.05 )。结论冰冻红细胞经第I 次洗涤液 (80 ml 9% NaCl) 的解冻洗涤过程为RBC回收率提高的关键步骤。 【关键词】冰冻红细胞 木糖醇 洗涤液 【中图分类号】 R33 1.1+41 【文献标识码】 A [文章编号】 1671-2587(2016)02-0149-04 Exploration on the Improvement of thawed Washing Solution of Frozen Red Blood Cells YU Lai-shui. KANG Wei, GONG Ben-lan. et al. Dalian Blood Centre, Dalian 116001 Abstract] Objective To explore the feasibility of improving the recovery ratio of red blood ce Ils in frozen- thawed red blood cells by adding an appropriate amount of xylitol during the washing process. Methods 20 packs of 400mL red blood cells which were leukocyte-reduced and prepared within 6 days were selected. The plasma was removed after centrifuge and each pack was divided into 2 bags (l 00 ml each) as experimental group and control group. In the experimental group , xylitol with final concentration of 1.2% ,0.2% was added into first washing solution (80 ml 9% NaCI) and second washing solution (200 ml 0.9% N aCI). The recovery ratio of red blood cells , residue level of glycerin and osmotic fragility in two groups were statisticaly analyzed. The key step of improving recovery ratio of red blood cells was sought by detecting the con