石蜡切片法(国外英文资料).doc

石蜡切片法(国外英文资料) Before the observation of fine structure of plants under the microscope, according to the characteristics of plant materials, using different methods, processing of plant material, the material is made of transparent glass specimen. According to the material properties and production method of different plants, electron microscopy techniques commonly used can be divided into two sections and nonslicing class, the former are the most commonly used hand sectioning, freeze sectioning, collodion slice method, paraffin section method, freezing method, the paraffin section method is most commonly used. The latter includes the whole sealed method, coating method, pressing method and so on. Paraffin sectioning Paraffin section method is based on the paraffin embedding agent, using a rotary microtome material will be sliced, into a permanent production through a series of treatment. In the study of plant cells, embryos and morphological aspects of paraffin section method is the most ideal method of production. The general process of paraffin section method for raw materials, fixed, pumping, washing, dehydration, transparent, paraffin embedding, repair, block, cutting, pasting sheet, dyeing and mounting. Sampling Cut the fresh plant material with a sharp blade and choose the right material. Fix it immediately Draw attention to the following: (1) take the material to be fresh. The action should be rapid, so as not to crush the damaged tissue. When the pathological material is taken, the contrast material should be set up (2) the material size should be appropriate: root and stem diameter less than or equal to 5mm, cut into 5-10mm segments; leaves generally take the midrib, cut into 2-5mm wide, 5-8mm long. In the cutting material, must pay attention to the blade axis at right angles, two section parallel sections, or made cells are skewed. Female, stamens generally does not require segmentation. (3) the sampling time according to the requirements of different sect