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中国农业科学 2008,41(5):1341-1346 Scientia Agricultura Sinica DNA微阵列技术在病原细菌基因转录谱分析中的应用 高世强，张新建，吴茂森，何晨阳 （中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室，北京 100094 ） 摘要：DNA 微阵列技术是近年来发展起来的一种功能基因组学研究技术。随着这项新技术的建立和日趋完善 及其在植物病理学研究中的应用，为病原细菌致病机理的研究带来了新思路并开辟了新途径。利用 DNA 微阵列技 术，检测病原细菌在不同生长条件下和在侵染植物过程中的基因转录谱，可以从基因组水平上鉴定出新的与毒性 和适应性相关的基因及其表达调控网络，有助于阐明这些基因的生物学功能及其机理。本文结合本实验室近几年 来的相关研究结果，综述DNA微阵列技术在病原细菌转录谱研究中应用的必威体育精装版进展。 关键词：DNA微阵列；植物病原细菌；基因表达谱；病原物—寄主互作；致病机制 DNA Microarray Technology and Its Application in Transcriptional Profiling of Bacterial Pathogens GAO Shi-qiang, ZHANG Xin-jian, WU Mao-sen, HE Chen-yang (State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094) Abstract: DNA microarray technique is one of the most powerful and innovative technologies for functional genomic researches. With the increasing development and application in plant pathology, the technique provides us the innovative idea and new insights into the pathogenic mechanisms of bacterial pathogens. Transcriptional profiling of the pathogens under in vitro and in vivo growth conditions revealed by DNA microarray analysis resulted in the identification of virulence- and adaptation-related genes and their regulatory networks, which is valuable to elucidate the biological functions of differentially-expressed genes. This paper has reviewed the latest progress in application of DNA microarray technology in the transcriptional profiling of pathogenic bacteria in combination with the related researches in authors’ laboratory in the past years. Key words: DNA microarray; Bacterial pathogens; Gene expression profiling; Pathogen-host interactions; Pathogenic mecha