胃癌患者胃液中特异性荧光物质初步确定-培训课件.ppt

Fig. 1. Discovery of fluorescence biomarkers. 图1 荧光标志物的发现 Fluorescence chromatograms (λ ex = 288 nm, λ em = 360 nm) of gastric juice from benign (c) and malignant (d) groups. 稀释胃液的荧光质谱图(激发光波长为288纳米，发射光波长为360纳米)，良性(c)和恶性(d)组。 Fig. 1. Discovery of fluorescence biomarkers. 图1 荧光标志物的发现 UV chromatograms (λ = 205 nm) of gastric juice from benign (e) and malignant (f) groups. 稀释胃液的紫外质谱图(激发光波长为205纳米)，良性(e)和恶性(f)组。 RESULTS The fluorescence intensity (FI), tyrosine, phenylalanine, tryptophan and total protein content were significantly higher in the?gastric juice?of patients with?gastric malignancies (all P0.01). 研究结果 胃恶性肿瘤患者胃液中酪氨酸、苯丙氨酸、色氨酸的荧光强度(FI)和血清总蛋白含量明显高于胃良性病变患者 (P值均0.01)。 Fig. 2. (a) Scatter plot indicates values of tyrosine, phenylalanine, tryptophan, total protein and FI in gastric juice from benign and malignant groups. 图2（a）散点图显示良性和恶性病变组患者胃液中酪氨酸、苯丙氨酸、色氨酸、总蛋白和荧光强度（FI）值。 RESULTS With all P0.001, the areas under the receiver operating characteristic curves of the biomarkers were tyrosine, 0.838; phenylalanine, 0.856; and tryptophan, 0.816. At a specificity of 79.4%, the sensitivity for?gastric?malignancy detection with phenylalanine was 87.9% only. 研究结果 在所有P 0.001的生物标志物中,受试者工作特征曲线（ROC）下面积分别是: 酪氨酸,0.838;苯丙氨酸,0.856;色氨酸,0.816。在特异性为79.4%时，苯丙氨酸用于胃恶性肿瘤检测的灵敏度为87.9%。 Fig. 2. (b) ROC curves for detection of gastric malignancies were plotted with the use of tyrosine (Tyr), phenylalanine (Phe), tryptophan (Trp), protein and FI. 图2（b）使用酪氨酸(Tyr),苯丙氨酸(Phe),色氨酸(Trp),总蛋白和荧光强度（FI）绘制的受试者工作特征（ROC）曲线用于检测胃恶性肿瘤。 DISCUSSIONS The elevation of aromatic amino acids in gastric juice may be explained as follows. First, malignant cells are extremely invasive, and they can synthesize and secrete many enzymes (e.g., matrix metalloproteinases, MMP and proteinases) that could degrade the basement membrane and extracellular matrix. 讨论 胃液芳香族氨基酸升高的机制如下： 首先，恶性细胞的侵袭性极强，它们可以合成并分泌许多酶类（例如，金属基质蛋白酶 MMP和蛋白酶）降解基底膜和细胞外基质。 DISCUSSIONS Collagen fibers and elastin contain endogenous fluorophores (aromati