三七不同药用部位有效成分含量测定.doc

三七不同药用部位有效成分含量测定 崔翰明 林海 路文龙 程慧平 张春光 王阶* 中国中医科学院广安门医院（北京市宣武区北线阁5号，100053） 摘要：目的 建立三七不同药用部位中三七皂苷R1、人参皂苷Rg1、Re和Rb1的HPLC含量测定方法，比较三七不同药用部位中上述4种皂苷的含量。方法 三七不同药用部位（主根、筋条、剪口、叶、花）甲醇提取，RP-HPLC/UV法测定，Kromasil C18 （250×4.6mm，5μm）色谱柱；以不同比例乙腈和水（v/v）线性梯度洗脱；流速：1mL·min-1；柱温：30℃；检测波长：203nm；进样量：10μL。结果 三七皂苷R1、人参皂苷Rg1、Re和Rb1可达到基线分离，其中人参皂苷Rg1和Re的分离度大于1.5；三七皂苷R1、人参皂苷Rg1、Re和Rb1的线性范围分别为：4.4-440μg·mL-1，4.32-1080μg·mL-1， 4.24-212μg·mL-1和4.48-1120μg·mL-1；4种皂苷的精密度和日间差RSD（n＝5）分别为0.46％，0.24％，0.77％，0.68％和1.64％，0.69％，0.52％，0.65％；加样回收率分别为103.26±1.22％，103.18±0.49％，103.20±1.58％，103.86±0.39％；三七不同药用部位含上述4种皂苷总量的顺序为剪口＞主根＞筋条＞叶＞花；而三七主根含上述4种皂苷总量的顺序为60头＞80头＞40头＞20头＞100头。结论 本方法简便、灵敏、准确，重现性良好，可用于准确分析三七不同药用部位中上述4种皂苷的含量。 关键词：三七；三七皂苷；高效液相色谱；含量测定 Abstract: Objective: A quantitative method was developed by gradient elution for the determination of notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in different position of Radix Notoginseng by HPLC. The content of 4 kinds saponins in different position of Radix Notoginseng was compared. Method: The different position of Radix Notoginseng (including root, rhizome, branch root, leaf, flower) were extracted with methanol. The HPLC conditions include Kromasil C18 column (250×4.6mm，5μm), different proportion acetonitrile and water linearity gradient elution, flow rate at 1.0mL·min-1, column temperature at 30℃, wavelength 203nm. Result: The linear ranges of notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 were 4.4-440μg·mL-1, 4.32-1080μg·mL-1, 4.24-212μg·mL-1 and 4.48-1120μg·mL-1, respectively. 4种皂苷的精密度和日间差RSD（n＝5）分别为0.46％，0.24％，0.77％，0.68％和1.64％，0.69％，0.52％，0.65％；加样回收率分别为103.26±1.22％，103.18±0.49％，103.20±1.58％，103.86±0.39％；三七不同药用部位含上述4种皂苷总量的顺序为剪口＞主根＞筋条＞叶＞花；而三七主根含上述4种皂苷总量的顺序为80头＞60头＞20头＞40头＞100头accurate and repeat, and was suitable in determination of the content of 4 kinds saponins in different position of Radix Notoginseng. method was sampa To structure. 三七为五加科Araliaceae人参属Panax植物三七Panax notoginsng (Burk) F.H.Chen。主产于我国云南、广西等省，为