阻塞性睡眠呼吸暂停综合征患者端粒长度的变化及意义.PDF

2011;31(3) 南方医科大学学报（J South Med Univ ） ·457 · ·临床研究· 阻塞性睡眠呼吸暂停综合征患者端粒长度的变化及意义 林 里，李涛平 南方医科大学南方医院呼吸科，广东广州510515 摘要：目的探讨阻塞性睡眠呼吸暂停综合征(OSAS)患者外周血白细胞端粒长度的变化及意义。方法 OSAS 患者11例， 健康对照组10例，提取外周血白细胞DNA ，纯度检测合格后，用Q-PCR 法检测端粒长度相对T/S 比率。结果 OSAS 组外 周血白细胞端粒长度相对T/S 比率较对照组小（P0.05）。结论 OSAS 患者外周血白细胞端粒长度的缩短可能与其发病 机制有关，低氧血症和高碳酸血症加速了端粒的损耗和细胞的凋亡。 关键词：阻塞性睡眠呼吸暂停综合征；端粒；白细胞 中图分类号：R563.1 文献标志码：A 文章编号：1673-4254（2011）03-0457-04 Alteration of telomere length of the peripheral white blood cells in patients with obstructive sleep apnea syndrome LIN Li, LI Tao-ping Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Abstract: Objective To study the alteration of telomere length of the peripheral white blood cells in patients with obstructive sleep apnea syndrome (OSAS) and explore its significance. Methods The DNA was extracted from the peripheral white blood cells of 11 patients with OSAS and 10 normal subjects matched for age and gender, and the T/S ratio was measured by fluorescence quantitative PCR. Results The T/S ratio in the peripheral white blood cells of patients with OSAS was obviously lower than that of the normal subjects (P0.05). Conclusion The reduction in the telomere length in the peripheral blood cells suggests a possible relationship between OSAS pathogenesis and telomere length, and hypoxemia and hypercapnia make accelerate telomere shortening and promote cell apoptosis. Key words: obstructive sleep apnea syndrome; telomere; white blood cells ［6-7 ］ 端粒是位于真核生物线性染色体末端的特殊序