反相C18液相色谱柱选择性理论与其应用_张伟清.pdf

药学学报 Acta Pharmaceutica Sinica 2010, 45 (5): 555−559 ·555 · 反相 C18 液相色谱柱选择性理论及其应用 * 张伟清, 胡昌勤 ( 中国药品生物制品检定所, 北京 100050) 摘要: 反相 HPLC 分析中色谱柱的选择性受到了越来越多的关注, 在这些色谱柱中, C18 柱是目前最常用的 色谱柱, 然而各种 C18 色谱柱在分离效果上也存在着较大差异, 因此能否找到合适的 C18 柱关系到整个实验的成 败。经过近年来的研究, 已形成了一些色谱柱分类、选择方法, 如色谱柱参数 (H , S, A , B , C) 法、相似系数 (F 值) 法等, 减少了色谱柱选择的盲目性。本文结合近年来的研究, 归纳了这些方法的优点及其在应用过程中的局 限性, 并基于前人的理论, 结合实际工作, 为药品分析中合理选择色谱柱提出了建议和方法。 关键词: 反相 HPLC; C18 柱; 选择性; 相似度 中图分类号: R917 文献标识码: A 文章编号: 0513-4870 (2010) 05-0555-05 Theory of selectivity of RP-LC C18 column and its application * ZHANG Wei-qing, HU Chang-qin (National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China) Abstract : In recent years, more and more researchers focus on the selectivity of RP-HPLC columns. Among these columns, C18 column is the most commonly used the column. Yet even the same filler can not give us the same separation effect with the columns of different brands and different types, therefore, it is important to choose the suitable HPLC column for carrying out an experiment. After study in recent years, some column classification, selection methods (e.g.: column parameter method, F value method) have been achieved and contributed to the finding of suitable columns and reducing the blindness of column selection. This paper