中国部分地区冠耳霉遗传多样性研究.PDF

安徽农业大学学报, 2011, 38(2): 238-243 Journal of Anhui Agricultural University [DOI]CNKI: 34－1162/S0938.001 中国部分地区冠耳霉的遗传多样性研究 李鲲鹏，王承芳，黄 勃* (安徽省微生物防治重点实验室，安徽农业大学，合肥 230036) 摘 要：为研究来自我国不同地区冠耳霉菌株的遗传多样性，用RAPD 和ISSR 分子标记，对来自中国安徽、 山东、贵州、山西和陕西5 省的 131 个冠耳霉菌株(Conidiobolus coronatus)进行遗传多样性分析。筛出的4 个RAPD 引物和3 个ISSR 引物共扩增出131 条带，其中多态性条带为 129 条，多态性比率为98.4% ，遗传相似性系数范围 在0.59~0.99 之间，说明受试的冠耳霉具有较为丰富的遗传多样性。利用RAPD 和ISSR 标记的结果，采用UPGMA 聚类分析方法可将绝大多数供试材料分为4 大类群。根据所划分的类群，发现冠耳霉之间的亲缘关系与地理来源存 在一定的相关性。研究表明，RAPD 和ISSR 标记可用于冠耳霉遗传多样性的研究。 关键词：冠耳霉；遗传多样性；RAPD；ISSR 中图分类号：Q949.323.4 文献标识码：A 文章编号：1672−352X (2011)02−0238−06 Genetic diversity of Conidiobolus coronatus from some regions of China LI Kun-peng, WANG Cheng-fang, HUANG Bo (Anhui Provincial Key Laboratory for Microbial Control, Anhui Agricultural University, Hefei 230036) Abstract: In order to study genetic diversity of Conidiobolus coronatus isolated from different geographical origin in China, genetic diversities of 131 C. coronatus isolates, randomly sampled from 5 Provinces （Anhui, Shandong, Gueizhou, Shanxi and Shanxi）in China, were analyzed using RAPD （random amplified polymorphic DNA ） and ISSR （inter-simple sequence repeat ）markers. By using 4 selected RAPD and 3 selected ISSR primers, 131 DNA bands were amplified, in which 129 bands showed polymorphism with a rate of 98.4%. The coefficient ranges of genetic similarity of the RAPD and ISSR markers were 0.59-0.99, which indicated that the abundant genetic diversities existed among the tested C. coronatus. UPGMA cluster analysis showed that these isolates were divided into 4 groups, and genetic similarities among C. coronatus isolates were partially related with geo- graphical sources. Based on the results, RAPD