Characteristics of a human cell line transformed by DNA from human adenovirus type 5-英文文献.pdf

J. gen. Virol. (2977), 36, 59-7z 59 Printed in Great Br#ain Characteristics of a Human Cell Line Transformed by D N A from Human Adenovirus Type 5 By F. L. G R A H A M * AND J. S M I L E Y t Departments of Biology and Pathology, McMaster University, Hamilton, Ontario, Canada W. C. R U S S E L L AND R. NAIRN;~ Divisions of Virology and Biochemistry, National Institutefor Medical Research, Mill Hill, London, NW7 IAA, U.K. (Accepted IO February I977) SUMMARY Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. The transformed cells (designated 293 cells) exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen. Analysis of the polypeptides syn- thesized in the 293 cells by labelling with 35S-methionine and SDS PAGE showed a variable pattern of synthesis, different in a number of respects from that seen in other human cells. On labelling the surface of cells by lactoperoxidase catalysed radio-iodination, the absence of a labelled polypeptide analogous to the 25o K (LETS) glycoprotein was noted. Hybridization of labelled cellular RNA with restriction fragments of adenovirus type 5 DNA indicated transcription of a por- tion of the adenovirus genome at the conventional left hand end.