Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation-英文文献.pdf

Published November 1, 1992 Identification of Programmed Cell Death In Situ via Specific Labeling of Nuclear DNA Fragmentation Yael Gavrieli,* Yoav Sherman,* and Shmuel A. Ben-Sasson* * Departments of Experimental Medicine and Cancer Research,* and Pathology, wThe Hebrew University-Hadassah Medical School, P. O. Box 1172, Jerusalem 91010, Israel Abstract. Programmed cell death (PCD) plays a key transferase, and then stained using avidin-conjugated role in developmental biology and in maintenance of peroxidase. The reaction is specific, only nuclei lo- the steady state in continuously renewing tissues. Cur- cated at positions where PCD is expected are stained. rently, its existence is inferred mainly from gel elec- The initial screening includes: small and large intestine, trophoresis of a pooled DNA extract as PCD was epidermis, lymphoid tissues, ovary, and other organs. D shown to be associated with DNA fragmentation. A detailed analysis revealed that the process is initi- o w Based on this observation, we describe here the devel- ated at the nuclear periphery, it is relatively short n l opment of a method for the in situ visualization of