胃康灵胶囊质量标准研究.doc

毕业论文 胃康灵胶囊质量标准研究 专 业： 中药学 目 录 中文摘要…………………………………………………………………………………1 英文摘要…………………………………………………………………………………2 1实验材料………………………………………………………………………………3 1.1药品与对照品………………………………………………………………………3 1.2仪器与试剂…………………………………………………………………………3 2薄层鉴别………………………………………………………………………………3 2.1甘草的薄层鉴别……………………………………………………………………3 2.2白及的薄层鉴别……………………………………………………………………4 2.3延胡索的薄层鉴别…………………………………………………………………4 2.4三七的薄层鉴别……………………………………………………………………5 3 质量标准研究…………………………………………………………………………6 3.1色谱条件的选择……………………………………………………………………6 3.2标准曲线的绘制与线性范围考察…………………………………………………8 3.3供试品溶液的制备…………………………………………………………………9 3.4阴性对照实验………………………………………………………………………10 3.5稳定性实验…………………………………………………………………………12 3.6重现性试验…………………………………………………………………………12 3.7加样回收率试验……………………………………………………………………13 4样品测定………………………………………………………………………………13 5 结果与讨论……………………………………………………………………………14 参考文献…………………………………………………………………………………15 致谢………………………………………………………………………………………16 综述………………………………………………………………………………………17  中文摘要 目的：研究并确定胃康灵胶囊的质量标准. 方法:采用TLC法及HPLC法对胃康灵胶囊进行鉴别和含量检测, 采用Diamonsil C18色谱柱:以乙腈-0.1%磷酸溶液(14:88)为流动相: 流速:1.0ml/min: 柱温:30℃: 检测波长为230nm 结果:按改进后的方法进行实验，杂质峰干扰较少，芍药苷分离度及拖尾因子较好，而且大大缩短了进样测试时间。同时在TLC鉴别中排除了阴性是否有干扰. 结论:TCL鉴别中阴性无干扰，含量测定改进方法后实验较满意，可以确定为其质量标准 关键词： 质量标准；薄层鉴别；高效液相 ABSTRACT Objective: To study and determine Weikangling capsule’s quality standards. Methods: TLC method and HPLC method were used for identification and testing of Weikangling capsule ,using Diamonsil C18 column, acetonitrile -0.1% phosphoric acid (14 : 88) at a flow rate: 1.0 ml / min, temperature: 30 ℃, detection at 230 nm. Results: by improved of the method of experiment, less interference-impurities, paeoniflorin isolation and tailing factor Better, but also shortened the sample testing time.and there is no interference in the TLC identification. Conclusion: TCL experients have no negative interference in the identification, determination of improving the methods of more satisfactory, so we can to determine it to be quality standards. Key