CACNA1F基因在3T3.docxVIP

CACNA1F基因在3T3 作者：刘勇 郭锡熔 潘晓勤 倪毓辉 陈荣华 【摘要】 目的 观察3T3-L1前体脂肪细胞诱导分化过程中L-型电压依赖钙离子通道α1F亚基(CACNA1F)基因表达水平的变化，探讨CACNA1F基因与肥胖发生的关系。方法 体外培养3T3-L1前体脂肪细胞，在诱导3T3-L1脂肪细胞分化成熟的不同时段，采用RT-PCR技术检测脂肪细胞中CACNA1F基因的mRNA表达水平。结果 CACNA1F基因在脂肪前体细胞中表达水平较低，随着脂肪细胞的分化成熟该基因表达水平逐渐升高，至分化第8～10天达到高峰。进一步的统计分析发现，CACNA1F基因的表达水平在细胞分化第0～3天、5～6天、8～10天3个时间段内无明显变化外(P＞0.05)，其余各时间段之间的表达水平差异均有显著性(P＜0.05)。结论 CACNA1F基因参与了脂肪细胞分化的调控，与肥胖发生相关，其在3T3-L1细胞分化过程中的表达变化可能有利于脂肪细胞的分化成熟和脂质积聚。 【关键词】 CACNA1F基因 3T3-L1前体脂肪细胞 细胞分化. 【Abstract】 Objective To investigate the changes of L-type voltage-dependent calcium channel α1F(CACNA1F)gene expression during 3T3-L1 preadipocyte differentiation.To explore the relationship between CACNA1F gene and the etiology of obesity.Methods 3T3-L1 preadipocytes were cultured in vitro and differentiated into the matured adipocytes.Total RNA was extracted from adipocytes of various times and the levels of CACNA1F gene mRNA expression were evaluated by RT-PCR.Results In preadipocye，the levels of CACNA1F gene mRNA expression remained at low.In the presence of inducing reagents，the CACNA1F gene mRNA expression was induced at a very early stage and remained at high levels in the mature adipocyte.There was a significant difference between any two detected phases in the levels of CACNA1F gene mRNA expression ，except that the levels of CACNA1F gene mRNA expression did not increase obviously in day 0 to 3 day，5th to 6th day，8th to 10th day.Conclusion CACNA1F gene is involved in the differentiation of adipocytes and related to the etiology of obesity.The changes in the level of CACNA1F gene mRNA expression during 3T3-L1 preadipocyte differentiation might be contributing to the differentiation and adipogenesis of adipocytes. 【Key words】 L-type voltage-dependent calcium channel α1F gene 3T3-L1 preadipocyte cell differentiation 近年来，我国儿童肥胖的发病率逐渐上升，而且与肥胖相关的一些疾病，如高血压、高脂血症、2型糖尿病等越来越多地出现在儿童群体中，肥胖已成为危害儿童身心健康的严重医学问题；然而肥胖病因复杂，是机体在遗传、环境、行为、生理、社会、文化等诸多因素相互作用下能量代谢失衡的结果，因此防治难度极大，一些传统的肥胖