DNA结合活性的影响.docxVIP

三七总皂甙对吗啡戒断大鼠海马磷酸化CREB蛋白表达及CREB/DNA结合活性的影响 作者：闫玉仙，呼文亮，丛斌，牛增强，李淑瑾，余磊，马春玲，张国忠，左敏 【关键词】 吗啡依赖 Effects of Panax Notoginseng on phosphorylated CREB protein expression and CREB/DNA binding activity in hippocampus of morphine withdrawal rats 　　【Abstract】 AIM: To investigate the effects of Panax Notoginseng (PNS) on the expressions of CREB, phosphorylated CREB (pCREB) and CREB/DNA binding activity in hippocampus of the morphine dependent rats and the naloxoneprecipitated withdrawal syndrome rats, and to explore the mechanism by which PNS inhibits the morphine withdrawal symptom in rats. METHODS: The models of morphine physical dependent rats and withdrawal syndrome were established by subcutaneous injection of morphine in gradually increasing doses and abdominal cavity injection of naloxone respectively. The rats were treated with PNS by intragastric administration at various doses simultaneously when administrated with morphine. The effects of morphine on the expressions of CREB, pCREB, CREB/DNA binding activity in hippocampus were detected by Western blot and electrophoresis mobility shift assay (EMSA). RESULTS: ①The CREB protein expression in hippocampus was not significantly statistically different in morphine physical dependent group (MOR), naloxone precipitated withdrawal group (NAL) and PNS groups compared with control group (Pgt;0.05); ②The pCREB protein expression and CREB/DNA binding activity in hippocampus were not significantly increased in MOR compared with control group and were significantly increased in NAL compared with MOR and control groups; ③PNS could dosedependently downregulate the increased expression of pCREB and CREB/DNA binding activity in hippocampus induced by naloxoneprecipitated withdrawal. CONCLUSION: PNS could inhibit the expression of pCREB and CREB/DNA binding activity in hippocampus in a dosedependent manner.　　【Keywords】 Panax Notoginseng; morphine dependence; naloxone; substance withdrawal syndrome; pCREB　　【摘要】 目的: 研究三七总皂甙（PNS）