肠微血管及体外培养内皮细胞通透性的影响及凋亡在其中的作用.docxVIP

内毒素对鼠肺/肠微血管及体外培养内皮细胞通透性的影响及凋亡在其中的作用 作者：贺志高1，肖南2 刘 韧2，田昆仑2，刁有芳2，范小青 【摘要】 目的 观察内毒素（LPS）对鼠肺和肠微血管及体外培养内皮细胞通透性的影响及凋亡在其中的可能作用。方法 (1)观察内毒素血症时大鼠肺、肠组织微血管通透性的改变。(2)观察LPS对体外培养人脐静脉内皮细胞（HUVECs）通透性的影响。(3)观察LPS对体外培养HUVEC凋亡的影响，从而探讨凋亡在内皮细胞通透性改变中的作用。结果 (1) 在大鼠内毒素血症模型中，5mg/kg和10mg/kg LPS均可造成肺、回肠组织伊文氏兰含量增加（Plt;0.01 或0.05）。(2)100μg/ml LPS可显著增加体外单层HUVEC对牛血清白蛋白的通透性（Plt;0.05或0.01）。(3)LPS可引起的体外培养内皮细胞的凋亡。结论 (1)LPS能迅速提高体内肺微血管内皮细胞通透性，而LPS作用后6h肠微血管通透性开始增加。(2)LPS可剂量依赖性的引起体外培养脐静脉内皮细胞通透性升高。(3)细胞凋亡可能是引起内毒素血症时内皮细胞通透性的原因之一。 【关键词】 内皮细胞通透性；内毒素 【Abstract】 Objective To observe the alteration of pulmonary and ileum microvascular permeability of rats and permeability alteration of monolayer endothelium in vitro following the administration of lipopolysaccharide， and the role of apoptosis in the altered permeability.Methods (1)To investigate the alteration of microvascular permeability of pulmonary and ileum in endotoxemia. (2)The effect of LPS on the permeability of human umbilicus vein endothelial cells (HUVECs) monolayer in vitro. (3)To observe the apoptosis of HUVECs following the administration of LPS. Results (1)The Evan’s blue content of pulmonary and ileum was increased after endotoxemia， but only the pulmonary reached significance(Plt;0.05 or 0.01). (2)When cocultured with HUVECs monolayer for 4h in vitro， LPS could dose-dependently increased leakage of FITC-BSA with significant effect at the dosage of LPS 100 μg/ml. Furthermore， LPS could induce endothelial apoptosis detected by acridine orange-ethidium bromide double stain and laser cofocal microscope.Conclusion (1)LPS significantly increased pulmonary microvascular permeability. (2)LPS could dose-dependently upregulate the permeability of monolayer endothelium in vitro.(3)LPS could result to the apoptosis of HUVECs in vitro and apoptosis might be one of the courses which result the upregulated endothelium permeability. 【Key words】 endothelial permeability；lipopolysaccharide histamine 内皮细胞通透性升高是炎性反应的重要特点，是微血管功能障碍