BtR05悬浮剂制备及其对南方根结线虫卵孵化的影响.pdf

26( ) 19- 23 Chinese Journal of Biolo ical Control 2010 11 BtR05 悬浮剂制备及其对南方根结线虫卵 孵化的影响 1 1 1 1 1 1 1, 2* 赵晓燕 , 李纪顺 , 黄玉杰 , 王贻莲 , 陈凯 , 扈进冬 , 杨合同 ( 1 / , 250014; 2 , 250014) 摘要: 通过对表面活性剂防腐剂悬浮剂的筛选和组合优化, 制备了苏云金芽胞杆菌B acillus thuring iensis BtR05 悬浮剂室 条件下储存 12 个月后, BtR05 悬浮剂的活菌存活率为 7474% 本试验测定了室内条件下BtR05 悬浮剂对南方根结线虫Meloidogyne incog nita 卵孵 化的抑制作用; 结果表明对南方根结线虫卵孵化的抑制率随悬浮剂浓度的增大以及处理时间 的延长而提高; BtR05 悬浮剂稀释25 倍液处理2d 和4d 后, 对南方根结线虫卵孵化的抑制率 分别为8567% 和9797% 关 键 词: BtR05 悬浮剂; 苏云金芽胞杆菌; 南方根结线虫; 卵孵化; 抑制率 中图分类号: S47611; S43245 文 标识码: A 文章编号: 2010) 增刊001905 Preparation of BtR05 Suspending Agent and Its Effect on Egghatching of Meloidogy ne incognita 1 1 1 1 1 ZHAO Xiaoyan , LI Jishun , HUANG Yujie , WANG Yilian , CHEN Kai , 1 1, 2* HU Jindon , YANG Heton ( 1Biotechnolo y Research Center of Shandon Academy of Science, Key Laboratory of Applied Microbiolo y of Shandon Province, Jinan 250014; 2 Institute of Biolo y, Shandon Academy of Sciences, Jinan 250014, China) Abstract: Surfactants, preservatives and suspendin a ents were screened and optimized to prepare sus pension concentrate of B acillus thuringiensis BtR05 The survival rate of BtR05 SC after 12 months preservation at room temperature was 7474%. The inhibitory effect of BtR05 SC on e hatchin of Meloidogyne incognita was tested, and the results showed that the inhibition rate of e hatchin of M . incognita was linearly related to the concentration of BtR05 SC. The inhibition rates of e hatchin after 2 d and 4 d treatment with 25 times dilution of BtR05 SC were 8567% and 9797% , respectively. Key words: BtR