C57BL6小鼠与BALBc小鼠对脓毒症反应的差异.PDF

2010;30(5) 南方医科大学学报(JSouthMedUniv) 窑973 窑 C57BL/6小鼠与BALB/c小鼠对脓毒症反应的差异 张婕袁刘占国袁罗宇维袁何懿袁顾冬生袁王明袁郑彦涛袁孙尔维(南方医科大学珠江医院器官移植科,广东广州 510282) 摘要院目的 探讨C57BL/6与BALB/c两种小鼠对脓毒症的反应能力遥 方法 C57BL/6小鼠和BALB/c小鼠各30 只袁分 别随机分成假手术组与脓毒症组袁每组 15只遥脓毒症组用盲肠结扎穿孔法渊CLP冤建立小鼠脓毒症模型遥术后6h袁每组 随机选出5 只袁Bio-plex悬浮蛋白芯片系统检测其外周血IL-1茁尧IL-2尧IL-4尧IL-5尧IL-10尧GM-CSF尧IFN-酌和TNF-琢 的 浓度遥剩余小鼠用于1周存活率分析遥结果 两品系小鼠假手术组 1周存活率均为100%遥C57BL/6小鼠脓毒症组1周 存活率为 10%袁BALB/c小鼠脓毒症组存活率为50%遥C57BL/6小鼠脓毒症组的存活率显著低于BALB/c小鼠脓毒症组 渊P0.05冤遥与假手术组相比袁C57BL/6小鼠脓毒症组分泌的IL-4尧TNF-琢和IL-10 明显升高遥而BALB/c小鼠脓毒症组分 泌的8种细胞因子较假手术组均未见明显增加遥 结论 C57BL/6 与BALB/c 两种小鼠对脓毒症的反应能力有差异袁 C57BL/6小鼠反应更敏感遥 关键词院C57BL/6小鼠曰BALB/c小鼠曰细胞因子曰脓毒症 中图分类号院R394;R631.2 文献标识码院 文章编号院1673-4254渊2010冤05-0973-03 DifferencesintheresponsetosepsisbetweenC57BL/6andBALB/cmice ZHANGJie,LIUZhan-guo,LUOYu-wei,HEYi,GUDong-sheng,WANGMing,ZHENGYan-tao,SUNEr-wei InstituteofTransplantImmunology,ZhujiangHospital,SouthernMedicalUniversity,Gangzhou510282,China Abstract:ObjectiveTocomparetheresponsestosepsisbetweenC57BL/6andBALB/cmice. MethodsThirtyC57BL/6mice and30BALB/cmicewererandomizedintosham-operatedgroupandsepsisgroup (n=15). Sepsismodelwasestablishedby cecalligationpuncture (CLP) inthemice, and6haftertheoperation, 5micefromeachgroupwereselectedrandomlyfor cytokinedetectionincludingIL-1茁,IL-2,IL-4,IL-5,IL-10,GM-CSF,IFN-酌andTNF-琢byBio-plex.Theother10miceineach groupwereusedforsurvivalanalysis. ResultsThesurvivalratesofBALB/candC57BL/6micewereboth100%inoneweek aftertheshamoperation,butloweredto10%and50%inoneweekafterCLP,respectively.ThesurvivalrateofC57BL/6mice wassignificantlylowerthanthatofBALB/cmice(P0.05).AfterCLP,C57BL/6miceshowedsignificantlygreaterIL-4,TNF-琢 andIL-10productionthanthesham-operatedmice,buttheconcentrationsofthe8cytokinesinBALB/cmiceafterCLPshowed nosignificantincrement.ConclusionComparedwithBALB/cmice,C57BL/6strainmouse