基础研究丹参改善骨骼肌缺血再灌注损伤的超微结构观察.PDF

2007 2 20 2 China J Orthop T raum a, Feb. 2007, Vo.l 20, No. 2 97 张俐, 洪振强, 鲁力, 陈伯仪 ( , 350003) ! : 通过透射电镜观察骨骼肌缺血再灌注损伤过程的病理生理变化探讨丹参的干预 用及机制 : 采用 90只清洁级雄性 SD大鼠, 6只 为健康对照组, 84只行左侧提睾肌主滋养动脉夹闭造模, 在缺血 25 h时, 丹参组腹腔注射丹参 ( 42只), 对照组注射等剂量的生理盐水 ( 42 只), 两组分别设缺血再灌注 102030405060 90 m in观测点 (每 个观测点 6只)观测结束后, 迅速切取提睾肌, 放入电镜固定液电镜下进行组织形态学观察 : 健康对照组正常情况下, 组成肌原纤维的粗细 2种肌丝沿其长轴排列, 每条肌原纤维上均可见明暗相间的带, 肌 浆内见有较多糖原颗粒及少量内质网线粒体等细胞器, 肌浆网纵向分布于肌原纤维周围, 线粒体膜及嵴清晰可见, 基 质着色深, 无肿胀空泡形成等改变, 在肌丝之间分布有较多糖原颗粒; 骨骼肌缺血再灌注后 102030405060 90 m in的 2组各观测点, 其病理变化较相似, 肌丝模糊凌乱, 部分溶解消失, 甚至完全消失, 肌浆网不同程度扩张, 线 粒体变大变圆, 基质变淡, 嵴变短变少甚至消失, 部分线粒体极度肿胀, 呈空泡状丹参组, 其病理变化大体相似, 肌纤 维均有不同程度恢复: 骨骼肌缺血再灌注损伤导致线粒体极度肿胀, 肌纤维紊乱丹参能有效地消除线粒体的 水肿, 恢复肌纤维, 减轻骨骼肌缺血再灌注损伤 ! 肌, 骨骼; 缺血; 再灌注损伤; 组织形态学; 透射电镜; 丹参 O b servation ofD ansh en () decoction attenua ting ischem ia reperfu sion injury of sk eleta l mu scle in rat model * ZHAN G L i , H ONG Zhenqiang, LUL i, CHEN Boyi. Departm ent of Orthopaedics and raumatology, Fuj ian College of rad i tional ChineseM edicine, F uzhou 350003, Fuj ian, China AB STRACT O bjective To evaluate the function ofDanshen ecoction in ischem ia/ reperfusion( I/R) injury by transm ission e lectron m icroscope. M e thods Six of the 90 m ale rats w ere selecte as norm al controls. The left crem aster muscles of 84 rats w eighting 250 g receive a stan ar iet an w ater were isolate an opene on the ventral s i e via an incision of the scrotum. Then the rats were ivi e ran om ly into 2 groups receiving a subcu taneous injection of eitherDanshen ecoction or the sam e volume of phosphate buffere saline as a contro.l Three hours of ischem ia was achieve by clam p ing them ain vascu lar pe icle. Pathologic features of the cremasterm uscle were ocum en te as in exs by transm ission electron m icroscope investigations. R e sults Norm ally, myogel