6-BA,NAA和Vc浓度配比对山葵试管苗增殖体系的影响-植物研究.PDF

生物技术通报 BIOTECHN OLOG YB ULLETIN 2006 6-BA, NAA Vc * 崔翠何凤发 周清元 (, 400716) : 山葵为十字花科山嵛菜属多年生草本植物, 具有强烈的香 味, 是 一种高级蔬菜和药用植物在山 葵生产中存在的一个最大问题即健壮种苗的供给, 通过山葵的茎尖培养建立组培快繁体系是解决该问题的有效手 段本文通过 311-A 最优回归设计研究 6-BA, NAA和 V c浓度配比对山葵试管苗增殖体系的影响, 对 11种不同配 方培养基中山葵苗的增殖系数进行调查, 通过 DPS3. 01数据统计软件建立回归方程, 并对回归方程进行主效因子 及其互作效应分析结果发现: 当 6-BA(X )NAA( X ) Vc(X )的浓度分别为 1. 2504m g/L0 m g/L1. 9668 m g/L 1 2 3 时, 增殖系数可以达到 6. 8380, 进一步优化了山葵试管苗增殖的体系 : 山葵 组织培养 311-A 回归设计 The Study on Improving Plantlet Propogation Coefficient of W a abi Through Optim izing Concentration of 6-BA, NAA and Vc C i C iHe Fengfa Zho Q ingy an ( Colleg e of A g ronom y and L if e Sc ience, Southw est Un iv ersity, Chong q ing 400716) Ab stract: W asab i (E utrema w asab ia (S ieb. ) M axmi . ) is am ember of cr ciferae. W asab i is a rarew orldw ide veg- etab le sed both for c linary and m ed icinal p rpose for its strong p ngent. How ever, one of the m ain problem s in plan ting w asabi is the prod ction of w asabi seedlings, in order to resolve efficiently th is prob lem, a shoot-tip c lt re system have been established. The st dy foc ses on mi proving plan tlet propogation, optmi ization regress analyse called 311-A was firstly app lied in w asabim ltiplication c lt re. The m ltip lication coefficients of 11 m ed i m w ith d ifferent level 6-BA and NAA and Vc w ere investigated. The regress eq ation was ded ced on 6-BA( X ), NAA (X ) and Vc(X ) as variab le factor, 1 2 3 and single factor effect and m