水产用聚维酮碘的安全性评价及其杀菌效果分析.pdfVIP

小抑菌浓度为 128~512 mg/L，对大型蚤的半数致死浓度为 13.44 mg/L。而且在养 殖水体中加入聚维酮碘至终浓度为 0.20~1.40 mg/L 后的 14 d 内，随着聚维酮碘浓 度的增加，各浓度组养殖水体的氨氮含量、亚硝酸盐含量均缓慢下降。 3 实验采用试管二倍稀释法，即将聚维酮碘分别加入含有 2 ml 无菌营养肉汤 的试管中，至终浓度分别为 0 mg/L、0.125 mg/L、0.25 mg/L、0.5 mg/L、1 mg/L、 2 mg/L、4 mg/L，8 mg/L、16 mg/L、32 mg/L、64 mg/L、128 mg/L、256 mg/L、512 mg/L、1 024 mg/L、2 048 mg/L、4 096 mg/L，并设 1 个阴性对照组，即空白无菌 8 营养肉汤组，分别接种各菌株的菌悬液至终浓度 3.0×10 cfu/ml，于 28℃恒温培养 24 h 后观察结果。把无菌生长、稀释度最大的聚维酮碘浓度作为各实验菌株的最 小抑菌浓度。从所有无细菌生长的各管中分别取 0.1 mL，涂布于相应的无菌琼脂 平板上，置于 28 ℃的恒温培养箱培养 24 h，观察结果。平板上无菌生长的最低质 量浓度为最小杀菌质量浓度。结果显示：聚维酮碘对弗氏柠檬酸杆菌(Citrobacter freundii)、鲁氏耶尔森氏菌(Yersinia Ruckeri)、副溶血弧菌（Vibrio Parahaemolyticus）、 嗜水气单胞菌(Aeromonas hydrophila)、溶藻弧菌（Vibrio alginolyticus）的抑菌和杀 菌效果不理想，聚维酮碘对铜绿假单胞菌（P.Aeruginosa）和哈维氏弧菌（Vibrio harveyi）的抑菌和杀菌效果较理想。 关键词：水产养殖，聚维酮碘，安全评价，抑菌效果 II Safety Evaluation, and Bactericidal effect of Fishery Povidonum-iodum ABSTRACT Povidone-iodine(PVP-I), as efficient disinfectant, widely used in aquaculture disinfection. However, with the rapid development of aquaculture, aquatic drugs extensive use of extensive and far-reaching impact on the aquatic environment, the environmental ecological problems resulting increasing peoples attention. In recent years, povidone-iodine to crabs, fish, shellfish, and shrimp etc. aquatic animals acute toxicity studies have been carried out, as well as the research of cytotoxic mechanism at home and abroad. From the existing literature, povidone-iodine in safety evaluation of the aquaculture environment rarely has been reported, resulting in there is a big blindness and one-sidedness of the aquaculture environment sa