146种培养基配方选读.docx

146种培养基配方THE COMPOSITION OF MEDIA?培养基及成分?1. Acetobacter Medium (醋酸菌培养基)?Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g?CaCO3 20g Agar (琼脂) 15g?Distilled water (蒸馏水) 1000ml Adjust (调) pH to 6.8?适用范围：恶臭醋酸杆菌混浊变种?2. Nutrient Agar (营养肉汁琼脂)?Pepton (蛋白胨) 5g Beef extract (牛肉膏) 30g?NaCl 5g Agar (琼脂) 15g?Distilled water (蒸馏水) Adjust (调) pH to 7.0-7.2?[Note]：When cultivation of Bacillus，5mg of to MnSO4.H2O may be added .?It is favorable to promote spore formation .?适用范围：产气气杆菌、粪产碱杆菌、蜡状芽孢杆菌、蜡状芽孢杆菌蕈状变种、地衣形芽孢杆菌、巨大芽孢杆菌、多粘芽孢杆菌、尘埃芽孢杆菌、短小芽孢杆菌、嗜热脂肪芽孢杆菌、枯草芽孢杆菌、枯草芽孢杆菌深黑变种、苏云金芽孢杆菌、苏云金芽孢杆菌蜡螟亚种（青虫菌）、苏云金芽孢杆菌戈尔斯德变种、苏云金芽孢杆菌猝倒亚种、产氨短杆菌、黄色短杆菌、谷氨酸棒状杆菌、北京棒杆菌、大肠埃希氏菌（大肠杆菌）、铜绿假单胞菌（绿脓杆菌）、凸形假单胞杆菌、荧光假单胞菌、弯曲假单胞菌、恶臭假单胞菌、假单胞杆菌、藤黄八叠球菌、亚黄八叠球菌、尿素八叠球菌、金黄色葡萄球菌、运动发酵单孢菌?3. Azotobacter Medium (固氮菌培养基)?KH2PO4 0.2g K2HPO4 0.8g?MgSO4.7H2O 0.2g CaSO4.2H2O　　 0.1ｇ?　　　Ｎa2MoO4.2H2O Trace(微量) Yeast axtract(酵母膏) 0.5g?Mannitol(甘露醇) 20g FeCl3 Tract(微量)?Distilled water (蒸馏水) 1000ml Agar (琼脂) 15g?Adjust (调) pH to 7.2?适用范围：固氮菌、胶质芽孢杆菌?4. Corn Meal Medium (玉米粉培养基)?Maize flour (玉米粉) 5g Peptone (蛋白胨) 0.1g?Glucose (葡萄糖) 1g Tap water (自来水) 1000ml?[Note]:Boil the mixture in autoclave at 121℃ for 1 hr. distribute the medium into 18ⅹ18 mm tubes , each contains 10 ml of the liquid , then autoclave at 121℃ for 1 hr . again (15磅蒸煮1小时,分装入18ⅹ18毫米试管,每管深度达6厘米。15磅再次灭菌15小时。)5. Lactic-bacteria Medium I (乳酸菌培养基 I )?Yeast extract (酵母膏) 7.5g Peptone (蛋白胨) 7.5g?Glucose (葡萄糖) 10g KH2PO4 2g?Tomato juice (西红柿汁) 100ml Tween (吐温) 80 0.5ml?Distilled water (蒸馏水) 900ml pH 7.0?适用范围：植物乳杆菌（胚芽乳杆菌）、嗜热乳酸链球菌?6. Lactic-bacteria Midium Ⅱ (乳酸菌培养基 Ⅱ)?Lacto-casein peptone (乳酪蛋白胨) 10g Beef extract (蛋白胨) 10g?Yeast extract (酵母膏) 5g Glucose (葡萄糖) 5g?Tween (吐温) 80 1g K2HPO4 2g?Na-acetate (醋酸钠) 5g Diamine citrate (柠檬酸二胺) 2g?MgSO4.7H2O 0.2g MnSO4.H2O 0.05g?Distilled water (蒸馏水) 1000m pH 6.5-6.8?适用范围：植物乳杆菌（胚芽乳杆菌）?7. Peotone Glucose Yeast extract Medium PGY （蛋白胨、酵母膏、葡萄糖培养基）?Peptone（蛋白胨） 10g Yeast extract （酵母膏） 5g?Glucose （葡萄糖） 1g Distilled water （蒸馏水） 1L?8. Glycerol Agar （甘油琼脂）?Pepto