DNALadder和ELISA联合检测PRRSV诱导Marc-四川农业大学学报.PDF

DOI :10.16036/j.issn.1000 -2650.2005.01.020 23 　 1 Vol.23 No.1 2005 3 　　　　　　　　 　Journal of Sichuan Agricultural University 　　　　　　　　 　 Mar.2005 Studies on Dynamic Changing Rules of Apoptosis in PRRSV-induced Marc 145 Cell Line Detected by DNA Ladder Combined with EL SA L I U W u-me i, WA NG Ming-shu , CHE NG A n-chun, L I U F ang, L I Xue-mei, Z HA NG Pi ng-y i ng , CHE N X iao-y ue (College of Animal and Technology, Sichuan Agricultural University, Yaan 625014, Sichuan, China) Abstract:For researching dynamic changing rules of a o tosis in PRRSV-induced M arc 145 cell line, sam les of M arc 145 cell line infected w ith PRRSV at 0, 24, 48, 72, 96, 120, 144 h and corres ond- ing time oints control M arc 145 cell line were collected, qualitative and quantitative a o tosis of cell cultures were detected by DNA Ladder and ELISA se arately.The result of DNA Ladder indicates that PRRSV can induce a o tosis of Marc 145 cell line in vitro and the DNA of cell cultures resented clas- sic Laddering attern.The result of ELISA indicates that M arc 145 cell begins to show a o tosis at 24 h ost-infected, a o tosis changes obviously at 48 h and the eak value of a o tosis a eares at 72 h , higher than that of control grou . Key words:DNA ladder;ELISA;PRRSV;a o tosis DNA Ladder ELISA PRRSV Marc 145 刘伍梅, 汪铭书 , 程安春, 刘　芳, 李雪梅, 张平英, 陈孝跃 (四川农业大学 动物科技学院, 四川雅安　625014) :对PRRSV 诱导Marc 145 细胞凋亡的动态变化规律进行了研究, 验收集PRRSV-SC1 株感染Marc 145 细胞 后0、24、48、72、96、120、144 h 和同一时间点未接毒的细胞样品, 用DNA Ladder 定性检测和ELISA 定量检测细胞凋 亡的变化规律。定性检测结果表明, PRRSV 能诱导Marc 145 细胞发生凋亡, 病毒感染后凋亡细胞 DNA 电泳呈典