三种细胞共培养微流控芯片的设计和制作.PDF

294 生 物 工 程 学 报 王帅超 等/三种细胞共培养微流控芯片的设计和制作 Chinese Journal of Biotechnology /cjbcn February 25, 2017, 33(2): 294−300 DOI: 10.13345/j.cjb.160300 ©2017 Chin J Biotech, All rights reserved ·组织工程与细胞培养· 1,2 2 2 2 1 1,2 王帅超 ，葛玉卿 ，吴蕾 ，郭慧 ，杨仕平 ，金庆辉 1 200234 2 200050 , , , . . , 2017, 33(2): 294–300. Wang SC, Ge YQ, Wu L, et al. Design and fabrication of a microfluidic chip for the co-culture of three cell types. Chin J Biotech, 2017, 33(2): 294–300. 摘 要: 设计一种具有“微坝”和“微缝”结构的微流控芯片，能够物理隔离不同细胞，而且培养基中小分 子营养物质可以自由流通。实验结果表明在芯片上可以共培养人肺腺癌细胞 (A549) 、人胚肺成纤维细胞 (HLF-1) 和人内皮细胞 (HUVECs) 三种细胞，在72 h 培养后三种细胞生长状态良好，具有细胞图形化的特点 和功能，为下一步开展多种细胞相互作用等相关研究提供重要的技术平台。 : 细胞共培养，微流控芯片，微坝和微缝结构 Design and fabrication of a microfluidic chip for the co-culture of three cell types 1,2 2 2 2 1 1,2 Shuaichao Wang , Yuqing Ge , Lei Wu , Hui Guo , Shiping Yang , and Qinghui Jin 1 College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China 2 State Key Laboratories of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China Abstract: Here a microfluidic chip with ‘micro-dam’ and ‘micro-gap’ has been designed and fabricated. It could isolate Received: August 10, 2016; Accepted: November 21, 2016 Supported by: National Basic Research Pro