单细胞分离检验技术在法医学中的应用研究.PDF

刑事技术 2015 年 第40 卷 第5 期 ·综  述· DOI ：10.16467/j.1008-3650.2015.05.003 单细胞分离检验技术在法医学中的应用研究 1 2 1 1 凃 1 1 1,* 丰 蕾 ，杨 帆 ，李彩霞 ，徐 珍 ， 政 ，李万水 ，胡 兰 （1. 公安部物证鉴定中心 法医遗传学公安部重点实验室，北京 100038 ；2. 上海市公安局刑侦总队，上海 200083） Single Cell Separation Testing and Its Forensic Application 1 2 1 1 1 1 1,*(1. Key Laboratory of Forensic Gene- FENG Lei , YANG Fan , LI Caixia , XU Zhen , TU Zheng , LI Wanshui , HU Lan tics of Ministry of Public Security, Institute of Forensic Science of Ministry of Public Security, Beijing , China; 2.Institue of 100038 Forensic Science, Shanghai Municipal Public Security Bureau, Shanghai 200083 , China) ABSTRACT: Forensic single cell separation testing technology is one of the most effective ways for trace and mixture DNA sample testing. Traditional single cell separation testing technology includes single cell isolation and lysis, PCR amplification and data analysis. This article details the principle of traditional single-cell separation and its application in case testing, meanwhile places the focus on prospect of single-cell sequencing (SCS) technology into forensic application. The research on SCS has shown tremendous growth over the past five years, and impacted many diverse areas of biological research. However, the study in forensic genetics is as yet waiting for developing. SCS is a combination of the single cell isolation, similar to the traditional single cell separation, with whole-genome amplification and next generation sequencing. In this review, the whole genome amplification and next generation sequenci