国人女性μ阿片受体A118G基因多态性与痛觉敏感性的关系.DOC

国人女性μ阿片受体A118G基因多态性对电刺激痛觉敏感性的影响 郑州大学第一附属医院麻醉科 450052 王中玉 张卫* 阚全程 常琰子 袁静静 储勤军 路辉 *通讯作者：张卫 教授，郑州大学第一附属医院麻醉科 450052 电子邮箱：zhangw571012@126.com 第一作者邮箱：wzy781217@163.com 论文类别：疼痛临床与基础研究 作者单位：450052，郑州大学第一附属医院麻醉科(王中玉、张卫、常琰子、袁静静、储勤军、路辉)；郑州大学第一附属医院临床药理基地 河南省高等学校临床医学重点学科开放实验室(阚全程) 【摘要】目的：研究国人女性μ阿片受体A118G基因多态性对电刺激痛觉敏感性的影响。方法：152例妇科择期子宫肌瘤剔除术或子宫全切手术患者，ASAⅠ或Ⅱ级，年龄20～50岁，体重指数在正常范围(1±20%)，采用电刺激测定患者痛阈和耐痛阈。使用聚合酶链反应-限制性片段长度多态性 (PCR-RFLP)技术检测 A118G基因型分布为：AA型76例，AG型56例，GG型20例。G等位基因频率为31.6%。三组间痛阈比较差异无统计学意义，GG组耐痛阈低于AA组和AG组。结论：国人女性G118等位基因频率为31.6%。A118G等位基因突变导致电刺激耐痛阈降低，本试验中A118G等位基因突变对痛阈无明显影响。 关键词：OPRM1；A118G；基因多态性；痛阈；耐痛阈 The effects of μ-opioid receptor gene polymorpohism A118G on experimental pain sensitivity with electric stimulation in chinese women WANG Zhong-yu, ZHANG Wei,KAN Quan-cheng, et al. Department of Anesthesiology，First Affiliated Hospital，Zhengzhou University，Zhengzhou 450052，China [Abstract] Objective: In this study we sought to determine the effects of μ-opioid receptor gene polymorpohism A118G on experimental pain sensitivity with electric stimulation in chinese women. Methods: 152 gynecological patients aged 20-50 yr (ASAⅠorⅡ, BMI is 1±20%) were divided into 3 groups according to genotype: group AA, AG and GG(Wild-type homozygous AA, heterozygous AG and mutant homozygous GG). The pain threshold and pain tolerance threshold were measured using electrical stimulation. All patients genotype were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for A118G polymorphism. Results: Genotyping analysis revealed that genotype distributions of the A118G polymorphism were 76 cases of AA, 56 cases of AG and 20 cases of GG. The G allele frequency were 31.6%. There were no difference in pain threshold among the three groups. But the pain tolerance threshold were significantly lower in group GG than in group AA and group AG. Conclusion: The G allele frequency is 31.6% and the A118G allele gene mutation induce to decreasing of pain toler