抗肝炎药双环醇研究(新).ppt

抗肝炎药双环醇的研究 中国医学科学院药物研究所 李 燕 升鹰榔鞋叮端打寅纷原钡氮增缘振黍唁谗累摄嚎啼硷恰召乃爸制殃迪觅芳抗肝炎药双环醇研究(新)抗肝炎药双环醇研究(新) 研究背景 现状 乙肝：HBsAg 1.2亿 乙肝患者 3000万 肝癌 30万/年 丙肝：3.2% Margolis et al. 1991（3.5亿) 抗病毒 抗肝损伤 抗纤维化 净饶喧浦盏毅娜熄荐焕敬夜芜槽卯惯等饯复俘铲碾谭由哎分今瞻缆犬峪畴抗肝炎药双环醇研究(新)抗肝炎药双环醇研究(新) 双环醇抗肝损伤作用及机制研究 撞篇叉要碳磺帅乓诬人诌交卤闰去倡义醒孰竞法绚埔昧些扛价芽一弱叠倪抗肝炎药双环醇研究(新)抗肝炎药双环醇研究(新) 双环醇对不同类型肝损伤的保护作用 Group Dosage mM GPT release U/106 cells GOT release U/106 cells MDA formation OD/106 cells Normal - 3.6±0.4 3.3 ±0.3 2.9 ±0.4 CCl4 - 10.2 ±2.2 7.0 ±0.5 6.8 ±2.4 Bicyclol 1.0 4.4 ±0.3** 3.8 ±0.2*** 1.6 ±0.5** 0.1 7.8 ±1.0 5.8 ±0.4*** 5.6 ±1.4** 0.001 8.4 ±0.8 6.0 ±0.2*** 5.8 ±0.9 肝细胞膜保护作用（形态学） 促进肝细胞蛋白合成 （同位素标记） Drug concentration 0 10-3M 10-4M Bicyclol 996±57 3053 ±29*** 1883 ±2*** Malotiate 893 ±59 1089 ±16* 1168 ±2** 降低升高的转氨酶（生化） 钒汽海尹涣呼薪贴坦惭署隙油逝短挡撇亚盅酬蛊贮均脑凄夫政桌究搽咎蓄抗肝炎药双环醇研究(新)抗肝炎药双环醇研究(新) 双环醇对化学性肝损伤的保护作用 血清氨基转移酶平均值（单位/dl) Effect of bicyclol on the elevation of serum aminotransferases and total bilirubin in LPS /GalN -intoxicated mice (n = 8). ##P0.01 vs control group, **P0.01 vs LPS /GalN group. Treatment Prothrombin time(s) Prothrombin activity (%) Normal 10.48±0.30 175±31 LPS /GalN 13.38±0.95## 67±13## Dexamethasone 10.58±0.45** 171±51** Bicyclol (300 mg/kg×3) 11.69±0.56** 105±21** Bicyclol (300 mg/kg×1) 12.23±0.62** 88±14** Effect of bicyclol on prothrombin time and prothrombin activity in LPS /GalN -intoxicated mice (n = 8) ##P0.01 vs normal group ; **P0.01 vs LPS/GalN group. Effect of bicyclol on the level of liver protein and glycogen in APAP-intoxicated mice (n =6). ##P0.01 vs control group; *P0.05, **P0.05 vs APAP group. (protein, g/L; glycogen, mg/g liver; FBG, mg/ml. ) 辅架陆植泛汉翼饮脂邢汉棺肺鞭粱灵钳魏若彻獭肃啼谜陋逊删气焰笼豢王抗肝炎药双环醇研究(新)抗肝炎药双环醇研究(新) 双环醇对化学性肝损伤的保护作用 Effect of bicyclol on the activity of liver ATPase in APAP-intoxicated mice(n=6). Liver specimens were collected at 16h after APAP injection. ##P0.01 vs control group. *p0.05 vs APAP group. A C D 15,000 B D F 30,000 A,B: 正常对