核磁共振技术在生物研究中的应用_姜凌.pdf

:60, , , 129 Xe , , , , , . * 姜 凌 刘买利 ( 430071) ( nuclear magnetic resonance, MR) . , Zeeman . Zeeman , , . , . , MR ,( ) ., MR . ,, , Nuclear magnetic resonance applications in biological systems JIA G Ling LIU MaiLi (StateK ey laboratory of Magnetic Resonance and A tomic and M olecular Physics, Wuhan Institute of Physics and Mathematics, ChineseAcademy of Sciences, Wuhan 430071, China) Abstract uclear magnetic resonance ( MR) spectroscopy is a stateoftheart technology which has been widely applied in biological systems over the past decades. It is a powerful tool for macromolecular structure determination in solution, and has the unique advantage of being capable of elucidating the struc ture and dynamic behavior of proteins during vital biomedical processes. In thisreview, we introduce the re cent progress in MR techniques for studying the structure, interaction and dynamics of proteins. The methods for MR based drug discovery and metabonomics are also briefly introduced. Keywords nuclear magnetic resonance( MR) ,protein solution structure, protein dynamics, drug screen 1945 MR ( Ernst, 1991 ; Wthrich , 2002 ,60 , MR ) , ( Lauterbur Mansfield, . 5 * ( : , ( : 2009CB918600) 2011- 02- 28 ( Rabi, 1944 ; Bloch Purcell, 1952 ) , . Email: ml.liu@wipm. ac. cn 366 http:www. wuli.ac. cn 40 (2011) 6 2003 ) . MR (EM) . , 12010 9 PDB , MR , 2007 , , 965 MR , . MR 13. 4%. MR