He-Ne 激光和增强UV-B 辐射对小麦幼苗多酚代谢3 种酶活性的影响.PDF

2009 11 17 6 Chinese Journal of Eco-Agriculture, Nov. 2009, 17(6): 1172−1175 DOI: 10. 3724/SP.J.1011.2009.01172 He-Ne UV-B 3 * 单 皓 韩 榕** ( 041004) 为探讨 He-Ne 激光和增强 UV-B 辐射对小麦幼苗多酚代谢相关酶活性的影响, 利用 5 mW ·mm−2 的 He-Ne 激光和增强 UV-B(10.08 kJ ·m−2 ·d−1)辐射处理“晋麦 8 号”小麦幼苗, 分别在不同时段测定苯丙氨酸 解氨酶(PAL) 、多酚氧化酶(PPO)及过氧化物酶(POD) 的活性。结果表明, 增强 UV-B 辐射使小麦幼苗叶片 PAL 活性升高, PPO 与 POD 活性降低, 且随处理天数的增加, 不同处理组小麦幼苗叶片 PAL 、PPO 、POD 活性间 具有一定相关性。长期增强 UV-B 辐射, 导致多酚类物质的代谢速度降低甚至停止, 自由基不能被及时清除, 生物膜破坏; 经过一定剂量的 He-Ne 激光辐照可修复增强 UV-B 辐射对小麦幼苗叶片多酚类物质代谢的损伤。 小麦幼苗 He-Ne 激光 UV-B 辐射 苯丙氨酸解氨酶 多酚氧化酶 过氧化物酶 : Q691.9 : A : 1671-3990(2009)06-1172-04 Effect of He-Ne laser and enhanced ultraviolet-B radiation on the activities of three polyphenol metabolism enzymes in wheat seedling SHAN Hao, HAN Rong (Institute of Cell Engineering, College of Life Sciences, Shanxi Normal University, Linfen 041004, China) −2 Abstract “Jinmai No. 8” wheat seedlings were exposed to He-Ne laser irradiation (5 mW·m ) and enhanced UV-B radiation (10.08 kJ·m−2·d−1) and the effect on the activities of three polyphenol metabolism enzymes (phenylalanine ammonialyase, polyphenol oxidase and peroxidase) in wheat seedling analyzed. The results show that UV-B radiation enhances wheat seedling phenylalanine ammonialyase activity while it limits activities of polyphenol oxidase and peroxidase. The activities among phenylalanine ammoni- alyase, polyphenol oxidase and peroxidase in different treatment groups get related with treatment time increased. The long-term enhanced UV-B radiation reduces polyphenol metabolic rate, breaking up biomembrane