湘鄂西地区珙桐天然群体遗传结构的研究.pdfVIP

第27卷 第6期 Vo1．27 No．6 2007年12月 Dcc．2OO7 文章编号：1673—923X(2007)06—0005—05 湘鄂西地区珙桐天然群体遗传结构的研究 徐刚标，禹玉婷，申响保 m (中南林业科技大学生命科学与技术学院，湖南 长沙 410004) 摘 要： 以珙桐叶为材料，对湘西和鄂西两天然珙桐群体的6o个样品进行RAPI)分析．结果表明，9个随机多态引物，获得24个多 态标记位点，平均每个引物产生2．67个多态位点；湘西和鄂西两群体之间的遗传多样性差异不明显，Shannon指数分别为0．427 4和 中 仃 a 0．448 1；种内平均遗传多样性为0．326 9，群体内平均遗传多样性为0．297 6，群体闻的遗传多样性为0．029 3，基因分化系数为 南 S o 0．089 6，基因流为5．079 2．两个天然珙桐群体的遗传相似性非常高，达到0．923 3，遗传距离为0．079 8；湘西群体的多态百分位率略高 林 于鄂西．这表明，湘西和鄂西两群体间存在着非常频繁的基因流． 业 U 关键词： 林学；珙桐；RAPD；自然群体；遗传结构；基因流 e 科 ． 中图分类号： s7l8．46 文献标志码： A 技 大 学 Studies of Genetic Diversity of Dove trees(Davidia involucrate) 』 r 学 in W est Hunan and Hubei by RAPD 报 ＆ C XU Gang—biao，YU Yu—ting，SHEN Xiang—biao (School of Life Science and Fcehnology．Central South University of Forestry and Technology，Changsha 410004，Hunan．China) 旧 y Abstract：Genetic diversity and population genetic structure in 60 individuals from two natural populations of Dove trees(Davidia involucrate)in West Hunan and West Hubei were studied by RAPD markers．24 polymorphic loci were obtained by 9 selected oIig()nuc【e()tlde primers．The genetic diversity between these populations was not obvious，and the Shannon information index was 0．427 4 and 0．448 1，respectively．The average genetic diversity within its species is 0．326 9．but 0．297 6 within the population and 0．029 3 between the populations．The coefficient of genetic differentiation was only 0．089 6 and gene flow has reached 5．079 2．The genetic ide