烟草赤星病拮抗细菌Ata28菌株的控病及促生效应.pdfVIP

★烟草科技 rI’0 ∞nn0∽n．eIIn 印rI’ nlII10—0氇 ★ ★病虫害防治 口．so∞∞o印Pe∞_ 0IItr0一★ 烟草赤星病拮抗细菌Ata28菌株的控病及促生效应 易 龙 ，严占勇 ，肖崇刚 1．西南大学植物保护学院，重庆北碚天生路216号400716 2．达州烟草专卖局，四川达州市通川区柴市街 18号635000 关键词：烟草赤星病菌；拮抗细菌；生物防治；促生作用 摘要：从烟草叶片中分离获得的对烟草赤星病菌[Ahernaria ahernata(Fries)Keissler]有明显抑 制作用的枯草芽孢杆菌Ata28菌株，经温室控病试验，能显著降低赤星病的发病率；将其用于 烟草种子的细菌化处理，以无菌土盆栽的方式测试其对烟草幼苗的促生效果，分别测定其地上 部鲜重，最大叶长、宽，真叶数。结果表明，拮抗细菌Ata28对烟草幼苗有明显促生效应。控 病、促生试验的Ata28菌液处理与对照间差异均达到显著水平，表明拮抗细菌Ata28兼具防病 作用和促生效应。 中图分类号：s482．2 92 文献标识码：B 文章编号：1002—0861(2007)12—0060—03 Growth-promoting and Control Effects of Strain Ata28 of Bacillus subtilis Against Alternaria alternata YI LONG(1)，YAN ZHAN—YONG(2)，and XIAO CHONG—GANG(1) 1．College of Plant Protection，Southwest University，Chongqing 4007 1 6，China 2．Dazhou Tobacco Monopoly Administration，Dazhou 635000，Sichuan，China Keywords：Ahernaria alternata；Antagonistic bacterium；Biocontrol；Growth—promoting effect Abstract：The strain Ata28 of Bacillus subtilis isolated from tobacco leaves could obviously decrease the occurrence of Ahernaria ahernata，which was proved by the disease control experiment in green— house．After seed bacterization，the growth—promoting effect of Ata28 on tobacco seedling was tested in pot with aseptic soil，and the fresh weight above ground，leaf number，and the length and width of the biggest leaf of tobacco seedling were determined．The results indicated that the growth—promoting effect of Ata28 was significant． The difference between Ata28 treatment and the control was significant， it showed that the antagonistic bacterium Ata28 possessed both seedling growth— promoting and disease control effec