离子交换高效液相色谱法测定淀粉类食品中丙烯酰胺含量的研究.pdfVIP

第25卷 第4期 四川农业大学学报 Vo1．25 No．4 2007年 l2月 Journal of Sichuan Agxicuhural University Dec．2007 Determination of Acrylamide in Starch·based Foods by Method of Ion·exchange HPLC dlANG Rong ，CHEN Yi—zi ，GENG Zhi—ruing ，CHEN Min82 (1．College of Infommtion and Engineering Technology，Sichuan Agricultural University，Yaan 625014，Sichuan，China； 2．Institute of )d Safety Research and Inspection，Jiangsu Academy of Agricultural Sciences，Nanjing 210014，China) Abstract：A simple and rapid method using ion—exchange high performance liquid chromatography (HPLC)coupled with diode array detection(DAD)WaS developed for the determination of acrylamide in starch—based foods．Acrylamide was extracted by 80％ methano1．and cleaned by a OaSis HLB solid． phaSe extraction(SPE)cartridge．For analysis，a HC一75H cation exchange column waS used with 0．005 mol／L sulfuric acid as mobile phaSe．flow rate was 0．6 mL／min and detection wavelength was set at 200 nm．The limit of detection was estimated at 30 ptg／kg，and recoveries of acrylamide from samples spiked at levels of l00～1000 ptg／kg ranged between 92％ and 104％．Finally．a test for com． mercial products such as potato crisps，biscuits，Youtiao，Shaobing showed good precision of the method ，with relative standard deviations below 9％，giving results similar with those reported in the literature． Key words：acrylamide；HPLC；SPE；starch．baSed food 离子交换高效液相色谱法测定淀粉类 食品中丙烯酰胺含量的研究‘ 蒋 荣 ，陈一资 ，耿志明 ，陈 明 (1．四川农业大学信息与工程技术学院，四川雅安 625014； 2．江苏省农业科学院食品质量安全与检测研究所，江苏南京 210014) 摘要：建立了淀粉类食品中丙烯酰胺含量的离子交换高效液相色谱测定方法。样品中的丙烯酰胺用80％甲醇溶液 提取，浓缩后经Oasis HLB固相萃取小柱净化，0．005mol 硫酸为流动相在HC一75H 阳离子交换柱上分离，二极 管阵列检测器(DAD)在200 nlTl波长进行定性、定量分析。检测限为30