硒对水分胁迫下赤霞珠葡萄幼苗叶片生理生化指标影响.doc

硒对水分胁迫下赤霞珠葡萄幼苗叶片生理生化指标影响摘 要： 研究硒对水分胁迫下赤霞珠葡萄幼苗叶片主要抗旱生理生化指标的影响，以期为探索硒元素在葡萄耐旱生产中的应用提供参考依据。以1 a生酿酒葡萄赤霞珠扦插苗为试材，开始水分胁迫后以清水喷施为对照，用0.5 mgL-1 （T1）、1.0 mgL-1（T2）、1.5 mgL-1（T3）的亚硒酸钠溶液喷施葡萄叶片正反面，定期采样测定葡萄叶片叶绿素、可溶性糖、脯氨酸、丙二醛（MDA）的含量，同时测定超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）活性。结果表明，整个水分胁迫期间，与CK相比，亚硒酸钠处理使葡萄叶片叶绿素含量平均提高2.93%～8.43%，可溶性糖含量平均提高9.7%～14.2%，脯氨酸含量提高12.0%～61.6%，SOD酶活性提高1.5%～6.6%，POD酶活性提高46.2%～87.8%，CAT酶活性提高18.5%～22.6%，GSH-Px酶活性提高14.5%～34.9%，同时使MDA含量降低10.1%～14.5%。综合分析认为，在该试验条件下对葡萄叶面喷施1.0 mgL-1的亚硒酸钠溶液，缓解水分胁迫对葡萄抗旱生理代谢带来的影响效果最佳。说明水分胁迫下，适宜浓度的硒可以缓解葡萄叶片叶绿素分解，提高可溶性糖及脯氨酸含量，同时可以调节SOD、POD、CAT、GSH-Px酶活性，降低MDA含量，抵御膜脂过氧化，提高赤霞珠葡萄幼苗的抗旱性 关键词： 赤霞珠葡萄； 硒； 水分胁迫； 生理生化指标 中图分类号：Ｓ６６3．1 文献标识码：Ａ 文章编号：１００９－９９８０?穴２０11?雪０6－984－０7 Effect of Selenium on physiological and biochemical indexes of Vitis vinifera cv. Cabernet Sauvignon leaves under water stress ZHAO Wei，XI Zhu-mei*，LIN Gang，TANG Jun-feng （College of Enology, Northwest Agricultural and Forestry UniversityShaanxi Engineering Research Center for Viti-Viniculture, Yangling， Shaanxi 712100 China） Abstract： The effect of Selenium on physiological and biochemical indexes of Cabernet Sauvignon under water stress was investigated to provide reference for drought tolerance cultivation of grape. The Vitis vinifera L. cv. Cabernet Sauvignon cuttings cultivated for one year were sprayed with Se-fertilizer under water stress at the Na2SeO3 concentrations of 0， 0.5， 1.0， 1.5 mgL-1. Grape leaves were sampled at special stage after Se-fertilizer spray to detect the content of chlorophyll， soluble sugar， proline and MDA as well as the enzyme activity of SOD，POD，CAT and GSH-Px. During the whole period of water stress， compared to CK the chlorophyll content was increased by 2.93% to 8.43% and soluble sugar content was increased by 9.7% to 14.2% in average after treatment， and the proline content was increased by 12.0% to 61.6%. While the SOD and POD activity was enhanced by 1.5% to 6.6% and 46.2% to 87.8%， respectively， and the CAT an