利用原子力显微镜观察芥蓝叶片气孔-湖南农业大学学报欢迎您！.PDF

第 36 卷第 1 期 湖南农业大学学报( 自然科学版) Vol.36 No.1 2010 年 2 月 Journal of Hunan Agricultural University (Natural Sciences) Feb ．2010 利用原子力显微镜观察芥蓝叶片气孔 1 2 2 陈少雄 ，陈家楠 ，蔡继业 (1.揭阳职业技术学院 生物系，广东 揭阳 522000 ；2.暨南大学 化学系，广东 广州 510632 ） 摘 要：利用原子力显微镜(atomic force microscope ，AFM) 的振荡模式研究芥蓝叶片上表皮气孔的三维形貌，并 对气孔在叶片失水过程中的形态变化进行动态观察．结果表明，通过 AFM 的扫描成像，观察到芥蓝叶片表皮的 气孔及其周围的细胞，包括保卫细胞、副卫细胞、其他表皮细胞等的微观结构，还观测到覆盖在副卫细胞的微纤 维，测量出这些微纤维的直径约为 400 nm ，间隔约为200 nm ． 关 键 词：芥蓝叶片气孔；原子力显微镜；形态学 中图分类号：Q24 文献标志码：A 文章编号：1007-1032(2010)01-0009-03 Morphological observation on stomata of cabbage mustard by atomic force microscope 1 2 2 CHEN Shao-xiong , CHEN Jia-nan , CAI Ji-ye (1.Department of Biology, Jieyang Vocational and Technical College, Jieyang, Guangdong 522000, China; 2.Department of Chemistry, Jinan University, Guangzhou 510632, China) Abstract ：The three-dimensional morphology was studied and the dynamic morphological changes in the process of losing water of stomata on the leaf of cabbage mustard were observed by using the tapping mode of atomic force microscope (AFM). The results showed that stomata and the other surrounding cells, including guard cells, subsidiary cells, and epidermal cells, could be clearly imaged by AFM. Furthermore, the micro fibers was found covering the subsidiary cells with diameter of about 400 nm with interval about 200 nm. Key words ：cabbage mustard stoma; atomic force microscope; morphology 高等植物叶片气孔的形态、数量以及分布与 孔表面的