四黄止痢颗粒中六味中药相互配伍对鸡大肠杆菌的作用-中国农学通报.PDF

中国农学通报 2012,28(14):89-92 Chinese Agricultural Science Bulletin 四黄止痢颗粒中六味中药相互配伍 对鸡大肠杆菌的作用研究 蒋 红，王宏军，周铁忠，刘英姿，刘智佳 （辽宁医学院，辽宁锦州121001） 摘 要：为了探讨四黄止痢颗粒中六味中药体外相互配伍抗菌活性，采用微管-平板法测定试验药物抗鸡 大肠杆菌的最小抑菌浓度(MIC) ，肉汤稀释棋盘法测定四黄止痢颗粒中六味中药相互配伍15 个药对抗 鸡大肠杆菌的协同指数(SI)及呈现协同作用时组方中各味中药的配比。结果表明：六味中药水提物中， 黄连抗鸡大肠杆菌的活性最强，MIC 为125 mg/mL ；配伍呈现协同作用的药对有4 对，其中黄芩与黄柏 配伍的SI 为0.27 ，配比为8:1，MIC 为31.3 mg/mL ；黄芩与黄连配伍，SI 为0.63 ，配比为1:2 ，MIC 为 62.5 mg/mL ；黄芩与甘草配伍的SI 为0.25 ，配比为1:4 ，MIC 为125 mg/mL ；黄芩与大黄配伍的SI 为0.38 ， 配比为1:2 ，MIC 为125 mg/mL 。本试验依据体外联合抗菌活性获得4 对中药配比，为四黄止痢颗粒复方 的拆方研究奠定基础。 关键词：中药；四黄止痢颗粒；鸡大肠杆菌；抗菌作用 中图分类号：S853.74 文献标志码：A 论文编号：2012-0339 The Combined Antibacterial Effect Study of Six Herbs in Sihuang Zhili Granules Against Chicken E. coli Jiang Hong, Wang Hongjun, Zhou Tiezhong, Liu Yingzi, liu Zhijia (LiaoningMedicalUniversity, Jinzhou Liaoning 121001) Abstract: In order to explore six herbs combined antibacterial activity in Sihuang Zhili granules against chicken E. coli in vitro. Minimum inhibitory concentration (MIC) of testing drug was detected against chicken E. coli by microtubule-plate method. The synergy index (SI) of fifteen Chinese drug pairs was detected by broth dilution checkerboard method in Sihuang Zhili granules and calculated the amount and ratio of herb couples when mutual compatibility of them showed synergism against chicken E. coli in vitro. The results showed that antibacterial effect of rhizoma coptidis was strongest in six herbs against chicken E. coli in vitro and MIC was 125 mg/mL. The combined antibacterial activity of four