6种中药活性成分植物雌激素作用的比较研究.pdfVIP

6种中药活性成分植物雌激素作用的比较研究 赵丕文，牛建昭 ，王继峰，王大伟，陈家旭，王玲巧(北京中医药大学基础医学院，北京100029) 摘要：目的 观察蜕皮甾酮等6种中药活性成分对MCF-7细胞增殖、细胞周期的影响，以探讨它们的植物雌激素样作用及其 可能机制。方法 采用M33细胞增殖实验观察6种中药活性成分：蜕皮甾酮、红花黄色素A、丹参酮I、丹参酮ⅡA、补骨脂素、 异补骨脂素对雌激素受体(ER)阳性细胞系MCF-7增殖的影响，并以雌激素受体拮抗剂ICI182，780干预，探讨其发挥雌激素 样作用的可能机制。同时，通过流式细胞术检测这些活性成分对MCF-7细胞增殖周期的影响。结果 蜕皮甾酮、红花黄色素 A、补骨脂素、异补骨脂素能够促进ER阳性细胞系MCF-7增殖，并将细胞周期由G 期向s期推进，促进DNA合成，提高细胞 分裂增殖指数。同时，加入ER拮抗剂ICI182，780可抑制其促增殖作用。丹参酮I和丹参酮ⅡA能够抑刺ER阳性细胞系 MCF-7增殖，但对其细胞周期影响不显著。同时，加入ER拮抗剂ICI182，780可减弱其对MCF-7增殖的抑刺作用。结论 蜕 皮甾酮、红花黄色素A、补骨脂素、异补骨脂素能够促进MCF-7增殖，而丹参酮I和丹参酮ⅡA可抑制MCF-7细胞增殖，其作用 均是通过ER介导的。 关键词：中药活性成分；植物雌激素；MCF-7；细胞增殖；细胞周期；ICI182，780 中图分类号：R285 文献标识码：A 文章编号：1001—2494(2007)24—1852—04 Phytoestrogenic Effects of Six Active Components in Chinese Medicine ZHAO Pi-wen，NIU Jian-zhao ，WANG Ji-feng，WANG Da-wei，CHEN a—XU，WANG Ling-qiao(Be~ing University ofTraditional Chinese Medicine，Belting 100029，China) AllSTRACT：OBJECTIVE To evaluate the estrogenic effects and their mechanisms of six active components in Chinese medicine through the test of their influence on MCF_7 proliferation and its cell cycle．METHoDS he proliferation of MCF-7 influenced by ecdysterone，saffcomin A，tanshinone I，tanshinone II A，psoralen and isopsoralen was analyzed by M33assay and their mechanisms were studied by adding ER antagonist ICI180．782．The cell cycle distribution of MCF-7 was determined by flow cytometry．RESULTS The proliferation rates of the cells treated with ecdysterone，saffcomin A，psoralen and isopsoralen were markedly increased．While the cell cycle was impulsed from G1 to S．DNA synthesis was inhanced and P1 was increased．The above function on boosting prolifera— tion was inhibited by adding estrogen receptor antagonism ICI1 82，780．Tanshinone I and tanshinone II A showed inhibitory effects on proliferation of ER positive MCF-7 cel1．However，no distinct effects were obtained on cell cycle．At the same time，the inhibitory effects of tanshinone I and tanshinone II A were signif