基于AngⅡ、ApoE表达探析补肾中药复方首参颗粒对颈动脉粥样硬化患者影响.docVIP

基于AngⅡ、ApoE表达探析补肾中药复方首参颗粒对颈动脉粥样硬化患者影响 　　[摘要] 目的 基于AngⅡ、ApoE的表达观察补肾中药复方首参颗粒对颈动脉粥样硬化患者的影响。 方法 选取2011年6月～2012年1月上海中医药大学附属市中医医院老年病科颈动脉粥样硬化患者80例为研究对象，采用区组随机化方法将其分为观察组（40例）和对照组（40例）。两组分别予以首参颗粒、普伐他汀口服治疗，疗程6个月。于治疗前、治疗后分别采用酶联免疫吸附测定（ELISA）法检测患者血清AngⅡ、ApoE表达。 结果 治疗后6个月，观察组AngⅡ表达较治疗前显著降低（P 0.05）；观察组ApoE表达较治疗前明显上升（P 0.05）。 结论 首参颗粒可能通过调控AngⅡ、ApoE的表达进而实现对动脉粥样硬化的多靶点干预 [关键词] 颈动脉粥样硬化；首参颗粒；AngⅡ；ApoE [中图分类号] R743 [文献标识码] A [文章编号] 1673-7210（2017）04（a）-0096-05 [Abstract] Objective To observe the effect of Chinese herbal formula for reinforcing kidney Shoushen Granules for the patients with carotid atherosclerosis based on the expression of AngⅡ and ApoE. Methods Eighty cases of patients with carotid atherosclerosis admitted to Department of Geriatrics， Shanghai Municipal Hospital of Traditional Chinese Medicine， Shanghai University of Traditional Chinese Medicine from June 2011 to January 2012 were selected as research objects， and they were divided into observation group （40 cases） and control group （40 cases） by method of block randomization. They were treated with Shoushen Granules or Pravastatin orally respectively for 6 months. Before and after treatment， the expression of AngⅡ and ApoE in serum was detected by enzyme-linked immunosorbent assay （ELISA） method respectively. Results Compared with before treatment， the expression of AngⅡ in the observation group after treatment was significantly decreased （P 0.05）. While the expression of ApoE in the observation group after treatment was significantly improved （P 0.05）. Conclusion Shoushen Granules achieve multiple target intervention of atherosclerosis may be through regulating the expression of AngⅡ and ApoE. [Key words] Carotid atherosclerosis； Shoushen Granules； AngⅡ； ApoE 动脉粥样硬化（AS）相关心脑血管疾病严重危害人类健康。补肾法是临床论治AS行之有效的中医治则[1]。课题组前期研究证实，补肾中药复方首参颗粒对颈动脉粥样硬化（CAS）患者可有效干预治疗[2-4]。基于此，本研究拟采用酶联免疫吸附测定（ELISA）法进一步观察CAS患者形成过程中血管紧张素Ⅱ（AngⅡ）、载脂蛋白E（ApoE）的变化以及首参颗粒的干预效果，以期为从补肾论治AS相关疾病提供深层次的临床依据 1 资料与方法 1.1 一般资料 选取2011年6月～2012年1月