B细胞和T细胞亚型在海人藻酸引起的C57BL／6鼠海马神经元损伤过程中不同的作用.pdfVIP

·706· J Apoplexy and Nervous Diseases，December 2007，Vol 24，No．6 文章编号：1003—2754(2007)06—0706—04 B 细胞和T细胞亚型在海人藻酸引起的C 5 7BL／6鼠 海马神经元损伤过程中不同的作用 崔香艳 ， 祝 威 ， 祝 捷 摘 要： 目的 应用海人藻阪在C57BI ／6免疫缺陷小鼠建立了神经退行性病变并观察了免疫活性B细胞和 T细胞亚型在病变过程中的作用。方法 经鼻滴人海人藻酸观察其临床和病理变化、细胞流式仪检测和分析脾细 胞表面标记。结果 海人藻酸引起了CD 基因敲除(CDr／一)、CDs(CDs／)、CD s(CD侣l／一)和B细胞(Igh6一／一)基因 敲 除鼠的临床抽搐症状千1l海码损伤。其临J术症状在CD (一／一)鼠最重，CD (一／一)，Igh6(一／一)以及野生型鼠次之，而 CD 、CD 双重基因缺陷鼠最轻。病理变化大约和临床症状相平行，脾细胞表面标记的表达也证实了上述发现。结论 获得性免疫反应参与r海人藻酸引起的海马损伤。CD T细胞和B细胞在病变过程中可能起到了保护作用，而 cD8T细胞则加重神经退行性病变。 关键词： 海人藻酸； 免疫缺陷鼠； 神经细胞； T细胞； B细胞 中图分类号：R741．02 文献标识码：A B cell and T cell subsets contribute differently to the pathogenesis that the KA—induced hippocampal neurOdegener— ation in C57BL／6 mice (771 Xtang—Jr“，2， H Wei，ZHU lie．(Department of Otorhinolaryngology in The first ttospital of Iilin University，(?hang~hllll 1 3002I，China) Abstract： Ob、jeetive To study the roles of B cell and T cell subsets in Kainic acid(KA)induced neurodegen— eration of hippocampus．Methods KA was administrated by intranasal route into C57／BI ／6 immune deficient mice．The clinical signs and the ncu roI)ath01()gjcal change of hippocampus as well as molecule expression were ob— served．Results CD!／CDH(一／一)mice had t11e mildest seizures；the responses of CD8(一／一)，Igh一6(一／一)and wild—type mice were intermediate．whereas CI)L(一／一)mice displayed much more severe clinical signs and 100 early mortali— ty．Hjstopatho1ogjca1 analysis of the mice that survived 7 days after KA administration revealed that CD{／CD8(一／一) mice had the fewest pathologic changes but that Igh一6(／一)mice showed more severe lesions in area CA3of the hip— pocampus than CI) (一／一)and wild type mice．Numbers of splenic CDlT cells were increased in CD8(一／一)and wild— type mice．CD s 1、cells wer