CFTR在超排卵幼龄大鼠卵巢的分布与表达.pdfVIP

476 咸宁学院学报(医学版)2007年第21卷第6期(Journal of Xianning College(Medical Sciences)] CFTR在超排卵幼龄大鼠卵巢的分布与表达 汤瑞玲 ，靳 镭 ’ (1．成宁学院附属第一医院妇产科，湖北成宁437100；2．华中科技大学附属同济医院生殖中心) 摘要：目的探讨囊性纤维化跨膜转导调节器(CrrR)在超排卵幼龄大鼠各期卵泡和黄体中表达的变化及 其意义。方法采用免疫组化和免疫印迹方法检测超排卵幼龄Wistar大鼠注射HCG前，注射HCG后24h、48h 及72h卵巢中CFTR的蛋白分布与含量变化。结果CFTR在超排卵幼龄Wist~大鼠的各级卵泡颗粒细胞、黄 体细胞均有表达，随着黄体期延长其表达量逐渐下降，各时段卵巢表达量比较有显著性差异。免疫印记法证 实各时段卵巢中均存在特异性的、相对分子质量为170KD的CFFR条带。结论 卵泡期CFFR表达可能促进卵 泡中卵泡液的积聚，卵泡直径增大，利于卵泡成熟；而黄体期CFFR表达下降则可能利于黄体形成，CFFR过多 表达可能导致卵泡囊肿和黄体囊肿。 关键词：囊性纤维化跨膜转导调节因子；卵巢；氯离子通道；大鼠 中图分类号：IB39．22 文献标识码：A 文章编号：1008-0635(2007)06．o476-04 Expression of Cystic Fibrosis Transmembrane Conductance Regulator in Rat Ovary TANG Rui—ling，JIN Lei (Department of Obstetrics and Gynecology，the First Affiliated Hosphal ofXianning College，Xianning Hubei 437100，China) ABSTRACT：Objective To investigated the expression of cystic fibrosis transmembrane conductance regu— lator(C兀R)，a cAMP—activated CI—channel，in ovarian stimulated premature female rat ovary during a cycle of follicle development and corpus luteum formation at protein leve1．Methods Animals were i．jected with 10 IU of PMSG and were given 10 IU of HCG 48h later．Time—course experiments were performed at Oh before，and 24， 48．72h after HCG treatment．Results Immunohistoehemistry showed that administration of PMSG stimulated CFTR expression in theeal cell layer and granulosa cell layer of mature follicles 48h post injection．coincident wit}I t}Ie PMSG—induced peak in follicular estradio1．However．the expression of CFrR was time—course reduced followed by HCG injection，in accordance with the gradually increased Plevel during luteum corpus formation． Western blotting analysis demonstrated that rat ovarian tissue expressed the special CF