川崎病小鼠模型心脏超声评价 - 欢迎访问中国比较医学杂志编辑部网站！.doc

川崎病小鼠模型超声评价 （1首都医科大学附属北京儿童医院，2中国医学科学院医学实验动物研究所，3美中互利医疗有限公司，北京，100045） 中文摘要 目的：干酪乳杆菌细胞壁提取物模型。将40只小鼠随机分为2组：KD模型组、20只。0d、21d和60dKD模型组LCWE注射后10d以及21d，超声心动图显示冠状动脉管壁与血管周围出现高回声，左冠状动脉内径值比较，KD模型组左冠状动脉内径值（10d:0.46±0.11 mm；21d: 0.47±0.09 mm）明显高于正常对照组小鼠（10d:0.32±0.14 mm；21d: 0.36±0.06 mm），差异具有明显统计学意义（10d:P=0.035；21d:P=0.027），4（20%）只小鼠出现冠状动脉瘤样扩张改变；造模后60d，KD模型组小鼠冠状动脉管壁弥漫性增厚，较前缩小（3±0.11 mm），与正常对照组（38±0.02 mm）比较无明显差异（P0.05）。 利用高频小动物心脏超声可获取小鼠清晰的冠状动脉图像，并能准确反映KD小鼠模型冠状动脉内径值动态变化；进一步证实LCWE诱导KD冠状动脉损伤与自然病程状态的KD患儿冠状动脉损伤情况【关键词】 川崎病；模型；超声心动图小鼠 【中分类号】P29 Assessment of echocardiography in a murine model of Kawasaki disease YANG Hai-ming1, DU Zhong-dong1, SHANG Guan-wen1, ZHANG Yan-lan1, DONG Wei2, SONG Ming-jing2, ZHENG Zhi-lan3 (1Department of Cardiology, Beijing Children’s Hospital, Capital Medical University,2Chinese Academy of Medical Sciences＆Comparative Medical Center, 3Chindex Medical limited company) Abstract Objective The aims of this study were to assess the developmental changes of coronary artery complications and Cardiac function in the murine model of Kawasaki disease (KD) With a high frequency ultrasound system and to provide evidence for preparation the murine model of KD. Methods Lactobacillus casei cell wall extract (LCWE) was prepared, and injected to C57BL/6 mice intraperitoneally to induce KD. Totally 40 mice were categorized into 2 groups randomly: KD model group and the control group with intraperitoneal injection of phosphate buffered solution, 20 mice in each group. On days 10, 21 and 60, the coronary artery dimensions were measured by echocardiography. Cardiac function including left ventricular end-diastolic diameter (LVIDd), left ventricular end-systolic diameter(LVIDs), ejection fraction(EF), fractional shortening(FS) and the blood flow velocity of atrioventricular valve during early diastole(E) /atrial contraction(A) were a