酸性橙Ⅱ半抗原及人工抗原的合成与鉴定-食品安全质量检测学报.PDF

6 8 Vol. 6 No. 8 2015 8 Journal of Food Safety and Quality Aug. , 2015 Ⅱ 1, 2 2 2 1 1, 2* 王 剑 , 王 萌 , 周兆祥 , 陈 琛 , 张小莺 (1. , 723001; 2. , 712100) 摘 要: 目的 Ⅱ方法 2- -5- 2-Ⅱ, (BSA)Ⅱ Ⅱ, Ⅱ结果 Ⅱ 47 mg, ⅡSDSN Ⅱ BSA, BSA, Ⅱ , Ⅱ, 20.9:1结论 Ⅱ , ⅡⅡ 关键词: Ⅱ; ; Synthesis and identification of acid orange Ⅱhapten and artificial antigen 1, 2 2 2 1 1, 2* WANG Jian , WANG Meng , ZHOU Zhao-Xiang , CHEN Chen , ZHANG Xiao-Ying (1. College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China; 2. College of Veterinary Medicine, Northwest AF University, Yangling 712100, China) ABSTRACT: Objective To explore a method for the synthesis and identification of acid orange Ⅱhapten and artificial antigen. Methods Acid orange Ⅱ hapten was synthesized by the reaction between 2-amino-5-sulfo-benzoic acid and 2-naphthol, then acid orange Ⅱ artificial antigen was prepared by active ester method. Acid orange Ⅱ hapten was identified by mass spectrometry and acid orange Ⅱ artificial antigen was identified by UV spectrophotometric, SDSas well as N. Results The structure of acid orange Ⅱ hapten was consistent with the predictive structure by