Mass Spectrometry - 质谱与微量分析实验室.PDF

Analytical Chemistry (2014) Chapter 21 – Mass Spectrometry Wang-Hsien Ding Ext: 65905 Office: S1-513 E-mail: wanghsiending@ What is mass spectrometry?  An analytical tool measuring molecular weight (MW) of sample  Only picomolar(10-12 M) concentrations required  Within an accuracy of 0.01% of total weight of sample (for a MW of 40 kDa, there is only a 4 Da error) and within 5 ppm (0.0005%) for small organic molecules  Ability of analyzing small and large molecules  It can detect structural modifications  Isotopic ratio of atoms in samples 1 Where are they used? Biotechnology: analysis of proteins, peptides, oligonucleotides Pharmaceutical: pharmokinetics, drugs discovery, combinatorial chemistry, drug metabolism Clinical: neonatal screening, haemoglobin analysis, drug testing Environmental: water, food, air quality (PCBs, Dioxins) Geological: oil composition The Components of Mass Spectrometer Mass Ion Ion source analyzer detector Vacuum pump Sample inlet See Box 21-1 2 電子束( ) (離子聚焦板) Magnetic sector MS See Box 21-2 3 Box 21-2 Ion Sources Gas-phase sourc