两种非甾体类抗炎药对清醒状态血管源性头痛模型大鼠颅内Fos表达的.DOC

两种非甾体类抗炎药对清醒状态血管源性头痛模型大鼠颅内Fos表达的影响 王晓辉1，朱浩静1，王恒1,孙宇1，吴士文1，于生元2 （1.武警总医院， 北京，100039；2.解放军总医院，北京，100853） 【摘要】目的：通过观察血管源性头痛清醒动物模型中Fos阳性细胞在三叉神经节及三叉神经脊束核尾侧亚核的分布情况，明确两种非甾体类抗炎药NSAID对乙酰氨基酚及布洛芬在头痛控制中的在颅内特定区域的作用机理。方法：30只雄性SD大鼠随机分为对照组（生理盐水组）、对乙酰氨基酚组、布洛芬组，每组给药后50分钟分别给予频率为20Hz、电流为3-5mA和脉宽为0.25ms的电刺激，刺激后给予大鼠灌注固定取脑，分别在颅内取三叉神经节及三叉神经脊束核尾侧亚核制作石蜡切片，进行免疫组织化学染色，利用Image J软件对阳性细胞进行计数统计。结果：电刺激后盐水组与非甾体类药物组在双侧三叉神经节、三叉神经脊束核尾侧亚核Fos蛋白表达的差异具有显著统计学意义，对乙酰氨基酚组与布洛芬组在双侧三叉神经节、三叉神经脊束核尾侧亚核Fos蛋白表达未见统计学差异。结论：给予非甾体类抗炎前后在双侧三叉神经节、三叉神经脊束核尾侧亚核的Fos表达的改变提示三叉神经节、三叉神经脊束核尾侧亚核参与了疼痛的传递和表达以及药物对疼痛控制的药理过程。 【关键词】偏头痛 动物模型 非甾体类抗炎药 对乙酰氨基酚 布洛芬 Fos蛋白 Effects of NSAIDs on Fos expression in conscious rat model of vasculogenic headache Wang xiao-hui1,Zhu hao-jing1, Wang heng1, Sun yu1, Wu shi-wen1,Yu sheng-yuan2, (1General Hospital of Armed Police Forces,Beijing,100039; PLA Postgraduate Medical School, Beijing 100853) 【Abstract】Objective By means of observing the distribution of Fos-immunoreactive neurons in trigeminal ganglia and trigeminal nucleus caudalis of conscious rat models of vasculogenic headache, define the functional mechanisms of the two NSAIDs paracetamol and ibuprofen in Specific brain regions in headache control. Methods 30 male SD rats were randomly divided into three groups:control group (saline group), acetaminophen group and ibuprofen group, each rat give electrical stimulation with parameter of frequency of 20Hz, 3-5mA current and pulse width of 0.25ms, after 50 minutes of the injection, perfusion fixation after electrical stimulation, trigeminal ganglia and trigeminal nucleus caudalis of the brains were taken out for paraffin sections, count the Fos-immunoreactive neurons with Image J by Immunohistochemical?staining .Results After electrical stimulation, there was significant difference of Fos protein expression in bilateral trigeminal ganglia, spinal trigeminal nucleus caudalis between saline group and group of non-steroidal anti-inflammatory drugs, but no significant difference of Fos protein expres