慢性应激性高血压动物模型的构建-第三军医大学学报.DOC

慢性复合应激诱发高血压动物模型的制备钟航海 (1.重庆市九龙坡区第五人民医院内科 40) 【摘要】 目的 应用多重刺激手段，模拟上班白领族遭受的常见复合应激因素，制备慢性应激性高血压大鼠模型，为进一步探讨SIH的发生、发展机制奠定基础。健康成年Wistar大鼠30只随机分为传统模型组（n=10）、自建模型组（n=10）及正常对照组（n=10）。传统模型组足底电击结合工业噪声复合刺激自建模型组应用不规则震荡、不良音乐、部分睡眠剥夺复合刺激法。正常对照组应激刺激。应激刺激第0、7、14、21、28日（分别以D0、D1、D7、D14、D21、D28）酶联免疫吸附法（ELISA）AngII）及皮质醇高效液相色谱（HPLC）法血浆儿茶酚胺含量传统模型组收缩压在应激刺激第7天较基础值上升约15 mmHg，在第14天达高峰[（140.6±4.3）mmHg]，而后略下降，但仍维持在高水平直至第28天；自制模型组SBP变化趋势与传统模型组类似，但上升时间及达高峰时间均晚1周；在观察期，正常对照组收缩压平稳。传统模型组及自制模型组心率在应激刺激1周后即开始增加，自第2周开始维持心率在较高水平，与正常对照组比较，差异有统计学意义（P0.05）。传统模型组及自制模型组血浆血管紧张素II、去甲肾上腺素、肾上腺素、皮质醇水平均显著高于正常对照组，差异均有统计学意义（P0.05）。不规则震荡、不良音乐、部分睡眠剥夺复合刺激法Establishement of chronic combined stimuli-induced hypertension animal model ZHONG Hang-hai1, FENG Ying-kai2. 1. Department of Internal Medicine, the Fifth People’s Hospital of Jiulongpo District of Chongqing City, Chongqing 400029, China; 2. Institute of Pathology, Westsouth Hospital of Third Military Medical University, Chongqing 400038, China 【Abstract】 Objective Multipe stimuli were adopted to simulate the common combined stressors from which the white-collar class suffers. The aim of the study is to develop chroinc sreess induced hypertension (SIH) rat model, so as to settle the basis for further investigation on occurrence and development of SIH. Methods Totally 30 healthy adult Wistar rats were randomly divided into traditional model group (n=10, receiving combined stimulation of electric foot shock and industrial noise), self-made model group (n=10, receiving combined stimulation of irregular shake, infaust music and partial sleep deprivation) and normal control group (n=10, no stimuli given). The modeling duration was 4 weeks. Systolic blood pressure (SBP) and heart rate (HR) were monitored at different time points (0, 7, 14, 21 and 28 days after first stimuation, which were denoted as D0, D1, D7, D14, D21 and D28 ) . On day 29, cervical arterial cannula was carried out to obtain the whole blood 4 mL; then the plasma was isolated. Enzyme linked immunosorbent a